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ORIGINAL RESEARCH article

Front. Microbiol.
Sec. Terrestrial Microbiology
Volume 15 - 2024 | doi: 10.3389/fmicb.2024.1471638

Microbial communities during the composting process of Agaricus subrufescens and their effects on mushroom agronomic and nutritional qualities

Provisionally accepted
Suyue Zheng Suyue Zheng 1*Chunxia Wang Chunxia Wang 1Dongxia Wang Dongxia Wang 2Chao Li Chao Li 3Zhaopeng Ge Zhaopeng Ge 1Liubin Hao Liubin Hao 1Fan Feng Fan Feng 1Yue Sun Yue Sun 1Yanfen Lyu Yanfen Lyu 1*
  • 1 Hebei University of Engineering, Handan, China
  • 2 China United Network Communications Co ., Ltd . Zhejiang Company, Hangzhou, China
  • 3 Chengde Academy of Agricultural and Forestry Sciences, Chengde, China

The final, formatted version of the article will be published soon.

    Tunnel composting technology for preparing Agaricus subrufescens cultivation media can achieve a higher biological efficiency (BE) and a lower contamination rate (CR). However, this technology lacks in-depth and systematic study. In the present study, the changes in the microbiome and microbial metabolic functions were surveyed using metagenomic analysis. The physicochemical parameters, agronomic properties and nutritional qualities were also evaluated. Results showed that the contents of cellulose, hemicellulose and lignin dropped to 10.18%, 11.58%, 27.53%, respectively at the end of composting. The tunnel composting technology led to significant increases in crude protein content (32.56%) and crude fiber content (13.68%). Variations of physicochemical characteristics led to different successions of microbial communities. Bacteria manifested significantly higher abundance than fungi. Firmicutes, Actinobacteriota, Chloroffexi and Deinococcota were the predominant bacterial phyla. Ascomycota and Basidiomycota were the dominant fungal phyla in the thermophilic phase. Pseudonocardia, Truepera and Thermopolyspora were positively correlated with the yield of A. subrufescens. In addition to TN, most of the physicochemical properties were significantly correlated with fungal communities in the thermophilic phase. The metabolisms of carbohydrate, amino acid and energy were the primary enrichment pathways. These findings deepen the understanding of microbial communities composition during the composting of A. subrufescens substrates. Moreover, this study provides a basis for improving tunnel composting technology.

    Keywords: composting, Agaricus subrufescens, microbial communities, high-throughput sequencing, metabolic function

    Received: 29 Jul 2024; Accepted: 26 Sep 2024.

    Copyright: © 2024 Zheng, Wang, Wang, Li, Ge, Hao, Feng, Sun and Lyu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Suyue Zheng, Hebei University of Engineering, Handan, China
    Yanfen Lyu, Hebei University of Engineering, Handan, China

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.