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ORIGINAL RESEARCH article

Front. Microbiol.
Sec. Microbe and Virus Interactions with Plants
Volume 15 - 2024 | doi: 10.3389/fmicb.2024.1469709
This article is part of the Research Topic Insights in Microbe and Virus Interactions with Plants: 2023 View all 11 articles

A PL1 family pectate lyase CP966_RS08110 gene was the pathogenic factors of Streptomyces galilaeus 5T-1 causing potato common scab

Provisionally accepted
Cuiwen Zhang Cuiwen Zhang Chengde Yang Chengde Yang *Mengjun Jin Mengjun Jin *Zhonghong Feng Zhonghong Feng *Richard Osei Richard Osei Fengfeng Cai Fengfeng Cai *Ting Ma Ting Ma *Yidan Wang Yidan Wang *
  • Gansu Agricultural University, Lanzhou, China

The final, formatted version of the article will be published soon.

    The previous results showed that the pectate lyase CP966_RS08110 gene of Streptomyces galilaeus 5T-1 was up-regulated in the pathogenic process. In this study, the CP966_RS08110 gene was cloned from the genomic DNA of S. galilaeus 5T-1. It encoded for a 415-residue protein with a complete PL-6 superfamily domain and Pec_lyase_C domain, which belongs to the PL1 family. The soluble protein encoded by CP966_RS08110 was obtained successfully, which has high pathogenicity after inoculating healthy potatoes. The mutant strain △PL5T-1 with CP966_RS08110 gene deletion was successfully obtained, and its colony morphology and pigment were not significantly different from that of wild strains, but its growth rate was slowed down, moreover, the hyaline circle formed by the mutant strain ΔPL5T-1 using pectin was significantly smaller than wild strain, and the deletion of this gene affected the infestation rate of S. galilaeus 5T-1. Our results confirm that the CP966_RS08110 gene was the pathogenic factors and played a key role in process of infecting and causing potato common scab, which laid foundation for understanding the pathogenic mechanism of S. galilaeus 5T-1.

    Keywords: Streptomyces galilaeus, Pectate lyase, Prokaryotic expression, Condition optimization, gene knockout

    Received: 24 Jul 2024; Accepted: 28 Oct 2024.

    Copyright: © 2024 Zhang, Yang, Jin, Feng, Osei, Cai, Ma and Wang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Chengde Yang, Gansu Agricultural University, Lanzhou, China
    Mengjun Jin, Gansu Agricultural University, Lanzhou, China
    Zhonghong Feng, Gansu Agricultural University, Lanzhou, China
    Fengfeng Cai, Gansu Agricultural University, Lanzhou, China
    Ting Ma, Gansu Agricultural University, Lanzhou, China
    Yidan Wang, Gansu Agricultural University, Lanzhou, China

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