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ORIGINAL RESEARCH article

Front. Microbiol.
Sec. Food Microbiology
Volume 15 - 2024 | doi: 10.3389/fmicb.2024.1468577

Improvement of silage characteristics of Lactobacillus salivarius HMC4 and improvement of silage quality of King grass

Provisionally accepted
Jinsong Yang Jinsong Yang 1Songsong Zhao Songsong Zhao 1Wenbo Zhi Wenbo Zhi 1Lu Tianyu Lu Tianyu 1化化 谯 化化 谯 1Lu Wei Lu Wei 1Ying Dou Ying Dou 1Haisheng Tan Haisheng Tan 1Hanlin Zhou Hanlin Zhou 1,2,3,4*
  • 1 Hainan University, Haikou, China
  • 2 Key Laboratory of Ministry of Agriculture and Rural Affairs for Germplasm Resources Conservation and Utilization of Cassava, Danzhou, Hainan, China
  • 3 Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China, Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan Province, China
  • 4 Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou, China

The final, formatted version of the article will be published soon.

    The effect of HMC4 produced by protoplast fusion on silage was studied. The silage formula was composed of heterozygote HMC4 (Group C), parent Lactobacillus (Group A) and a combination of two parents (Group B). The fermentation quality and microbial composition of each batch of silage were evaluated. The results showed that the propionic acid concentration in group C was the lowest, but the organic acid content in feed was significantly increased. Groups B and C had slightly lower crude fiber levels than group A, while groups A and C had higher levels of soluble sugars.The dynamic observation of C group showed that the nutrient composition of feed changed gradually with the extension of silage period. High-throughput sequencing revealed fluctuations in microbial composition before and after silage. Over time, Lactobacillus became the dominant strain and its numbers steadily increased.

    Keywords: protoplast fusion, Silage, Lactobacillus, high-throughput sequencing, microbial communities

    Received: 07 Aug 2024; Accepted: 07 Nov 2024.

    Copyright: © 2024 Yang, Zhao, Zhi, Tianyu, 谯, Wei, Dou, Tan and Zhou. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Hanlin Zhou, Key Laboratory of Ministry of Agriculture and Rural Affairs for Germplasm Resources Conservation and Utilization of Cassava, Danzhou, Hainan, China

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