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ORIGINAL RESEARCH article

Front. Microbiol.
Sec. Infectious Agents and Disease
Volume 15 - 2024 | doi: 10.3389/fmicb.2024.1464444
This article is part of the Research Topic One Health Approach to Mycobacterial Infections in Veterinary Science View all 3 articles

Novel target and cofactor repertoire for the transcriptional regulator JTY_0672 from Mycobacterium bovis BCG

Provisionally accepted
Hui Wang Hui Wang 1Xiaotian Li Xiaotian Li 1Shuxian Wang Shuxian Wang 1Ren Fang Ren Fang 1Jiayin Xing Jiayin Xing 1Ruiying Wu Ruiying Wu 1Chunhui Zhang Chunhui Zhang 1Zhaoli Li Zhaoli Li 2Ningning Song Ningning Song 1*
  • 1 Shandong Second Medical University, Weifang, China
  • 2 SAFE Pharmaceutical Technology Co. Ltd, Beijing, China

The final, formatted version of the article will be published soon.

    Mycobacterium tuberculosis (Mtb) is the pathogenic agent of tuberculosis (TB). Intracellular survival plays a central role in the pathogenesis of Mtb in a manner that is dependent on an array of transcriptional regulators for Mtb. However, the functionality of JTY_0672, a member of the TetR family of transcriptional regulators, remains unknown. In this study, we found that the transcriptional regulator JTY_0672 is a broad-spectrum transcriptional regulatory protein and can directly regulate JTY_3148, both in vitro and in vivo. Cofactors containing VB1, VB3, VB6, VC, His, Cys, Asp, Glu, Fe 3+ , Pb 2+ , Cu 2+ , and Li + were found to inhibit binding between JTY_0672 and the promoter of JTY_3148.JTY_0672 enhanced TAG production and increaed Isoniazid (INH) resistance. Besides, this protein either promoted recalcitrance to the host immune response and induced pathological injury and inflammation. In summary, this research identified new targets and cofactors of JTY_0672 and deciphered the physiological functionality of JTY_0672. Our findings will provide an important theoretical basis for understanding the Mtb transcriptional regulatory mechanism.To survive, Mtb have to face a range of stress factors such as temperature (Gauthier et al., 2021,

    Keywords: Mycobacterium tuberculosis, Transcriptional regulation, JTY_0672, JTY_3148, cofactor

    Received: 14 Jul 2024; Accepted: 13 Dec 2024.

    Copyright: © 2024 Wang, Li, Wang, Fang, Xing, Wu, Zhang, Li and Song. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Ningning Song, Shandong Second Medical University, Weifang, China

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