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METHODS article

Front. Microbiol.
Sec. Microbiotechnology
Volume 15 - 2024 | doi: 10.3389/fmicb.2024.1463645
This article is part of the Research Topic Development of Functional Bacteria and its Application in Silage Processing View all 5 articles

A slow-release strategy of Lactobacillus plantarum to enhance the degradation of cellulose by Bacillus methylotrophic in the ensiling process of corn stalk

Provisionally accepted
思源 刘 思源 刘 yongqing wan yongqing wan yunhao song yunhao song ruihua tian ruihua tian ruigang wang ruigang wang kaihong duan kaihong duan Mandlaa Mandlaa Mandlaa Mandlaa *
  • Inner Mongolia Agricultural University, Hohhot, China

The final, formatted version of the article will be published soon.

    The enhancement of cellulose degradation is important for improving the quality of corn-stalk silage. However, the rapid drop of pH caused by the propagation of lactic acid bacteria (LAB) can influence the degradation of cellulose by cellulose-degrading microorganism (CDM) during mixed-fermentation process of ensilage. In this study, a CDM (Bacillus methylotrophic 2-4, BM 2-4) was isolated and the lyophilization condition of it was studied. Moreover, a slow-release strategy was developed to delay the release of LAB (Lactobacillus plantarum S-1, LP S-1) by embedding technology to provide time for BM 2-4 to degrade cellulose during the corn-stalk ensilage process. The results showed that BM 2-4 had a higher survival rate (89.53%) under the condition of cell collection (5600 r/min in 4.4℃ for 9.5 min centrifugation) and lyophilization (cryoprotectants: skim milk 10.4 %, peptone 10.4 % and glucose 5.2 %; -50℃ and vacuum degree<10 Pa). Based on the results of the previous study about embedded LP S-1, simultaneous inoculation of embedded LP S-1 and lyophilized BM2-4 under the conditions of proportion 2:1, inoculum size 6% and moisture 85% could significantly degrade CF by 3.8 % and increase CP by 3.7 %(p<0.05) without significant influence on the final pH of corn-stalk silage (p>0.05) after 7 days fermentation.

    Keywords: Corn stalk, Silage, Slow-release, Bacillus methylotrophicus, Lactobacillus plantarum

    Received: 12 Jul 2024; Accepted: 15 Nov 2024.

    Copyright: © 2024 刘, wan, song, tian, wang, duan and Mandlaa. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Mandlaa Mandlaa, Inner Mongolia Agricultural University, Hohhot, China

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