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ORIGINAL RESEARCH article

Front. Microbiol.
Sec. Microbe and Virus Interactions with Plants
Volume 15 - 2024 | doi: 10.3389/fmicb.2024.1461988
This article is part of the Research Topic Plant Viruses and Viroids: Molecular Biology, Ecology and Pathogenicity View all articles

Identification and Molecular AnalysisMolecular Detection and Sequencing of Beet Necrotic Yellow Vein Virus and Beet Cryptic Virus 2 in Sugar Beet from Kazakhstan

Provisionally accepted
Alexandr Pozharskiy Alexandr Pozharskiy Aruzhan Mendybayeva Aruzhan Mendybayeva Ruslan Moisseyev Ruslan Moisseyev Marina Khusnitdinova Marina Khusnitdinova Dilyara Gritsenko Dilyara Gritsenko *Gulnaz Nizamdinova Gulnaz Nizamdinova
  • Institute of Plant Biology and Biotechnology (IPBB), Almaty, Kazakhstan

The final, formatted version of the article will be published soon.

    Beet necrotic yellow vein virus (BNYVV) is a common viral pathogen that causes considerable economic loss globally. In the present study, a commercial real-time PCR test system and custom loop mediated amplification primers were used to detect the virus in asymptomatic sugar beet samples. A total of 107 of 124 samples tested positive for the presence of the A type A BNYVV coat protein gene. Wide coverageNear complete sequences of RNA-3 and RNA-4 were obtained using reverse transcription, followed by nanopore sequencing of 14 isolatessamples. A comparison with available sequences, including previously published isolates Kas2 and Kas3 from Kazakhstan, identified RNA-3 as belongingsimilar to such of the P -type isolates Puthiviers and Kas3. RNA-5 was not detected using real-time PCR or cDNA amplification. Unique variable sites were identified in the p25 protein sequence translated from RNA-3. Another virus, beet cryptic virus 2 (BCV2), was identified and sequenced in samples infected with BNYVV. With 85.28% genome coverage, the identified BCV2 isolatessamples were very similar to the previously reported isolates from Hungary and Germany.

    Keywords: BNYVV, Polymyxa betae, rhizomania, BCV2, LAMP

    Received: 09 Jul 2024; Accepted: 23 Oct 2024.

    Copyright: © 2024 Pozharskiy, Mendybayeva, Moisseyev, Khusnitdinova, Gritsenko and Nizamdinova. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Dilyara Gritsenko, Institute of Plant Biology and Biotechnology (IPBB), Almaty, Kazakhstan

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