AUTHOR=Rowe John C. , Summers Stacie C. , Quimby Jessica M. , Winston Jenessa A. 

TITLE=Fecal bile acid dysmetabolism and reduced ursodeoxycholic acid correlate with novel microbial signatures in feline chronic kidney disease

JOURNAL=Frontiers in Microbiology

VOLUME=15

YEAR=2024

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2024.1458090

DOI=10.3389/fmicb.2024.1458090

ISSN=1664-302X

ABSTRACT=<sec id="sec1"><title>Background</title><p>Microbial-derived secondary bile acids (SBAs) are reabsorbed and sensed via host receptors modulating cellular inflammation and fibrosis. Feline chronic kidney disease (CKD) occurs with progressive renal inflammation and fibrosis, mirroring the disease pathophysiology of human CKD patients.</p></sec><sec id="sec2"><title>Methods</title><p>Prospective cross-sectional study compared healthy cats (<italic>n</italic> = 6) with CKD (IRIS Stage 2 <italic>n</italic> = 17, Stage 3 or 4 <italic>n</italic> = 11). Single timepoint fecal samples from all cats underwent targeted bile acid metabolomics. 16S rRNA gene amplicon sequencing using DADA2 with SILVA taxonomy characterized the fecal microbiota.</p></sec><sec id="sec3"><title>Results</title><p>CKD cats had significantly reduced fecal concentrations (median 12.8 ng/mg, Mann–Whitney <italic>p</italic> = 0.0127) of the SBA ursodeoxycholic acid (UDCA) compared to healthy cats (median 39.4 ng/mg). Bile acid dysmetabolism characterized by &lt;50% SBAs was present in 8/28 CKD and 0/6 healthy cats. Beta diversity significantly differed between cats with &lt;50% SBAs and &gt; 50% SBAs (PERMANOVA <italic>p</italic> &lt; 0.0001). Twenty-six amplicon sequence variants (ASVs) with &gt;97% nucleotide identity to <italic>Peptacetobacter hiranonis</italic> were identified. <italic>P. hiranonis</italic> combined relative abundance was significantly reduced (median 2.1%) in CKD cats with &lt;50% SBAs compared to CKD cats with &gt;50% SBAs (median 13.9%, adjusted <italic>p</italic> = 0.0002) and healthy cats with &gt;50% SBAs (median 15.5%, adjusted <italic>p</italic> = 0.0112). <italic>P. hiranonis</italic> combined relative abundance was significantly positively correlated with the SBAs deoxycholic acid (Spearman <italic>r</italic> = 0.5218, adjusted <italic>p</italic> = 0.0407) and lithocholic acid (Spearman <italic>r</italic> = 0.5615, adjusted <italic>p</italic> = 0.0156). Three <italic>Oscillospirales</italic> ASVs and a <italic>Roseburia</italic> ASV were also identified as significantly correlated with fecal SBAs.</p></sec><sec id="sec4"><title>Clinical and translational importance</title><p>The gut-kidney axis mediated through microbial-derived SBAs appears relevant to the spontaneous animal CKD model of domestic cats. This includes reduced fecal concentrations of the microbial-derived SBA UDCA, known to regulate inflammation and fibrosis and be reno-protective. Microbes correlated with fecal SBAs include <italic>bai</italic> operon containing <italic>P. hiranonis</italic>, as well as members of <italic>Oscillospirales</italic>, which also harbor a functional <italic>bai</italic> operon. Ultimately, CKD cats represent a translational opportunity to study the role of SBAs in the gut-kidney axis, including the potential to identify novel microbial-directed therapeutics to mitigate CKD pathogenesis in veterinary patients and humans alike.</p></sec>