The final, formatted version of the article will be published soon.
ORIGINAL RESEARCH article
Front. Microbiol.
Sec. Food Microbiology
Volume 15 - 2024 |
doi: 10.3389/fmicb.2024.1456461
Response Surface Methodology-Based Optimization of Inonotus hispidus' Liquid Fermentation Medium and Evaluation of Its Exopolysaccharide Activities
Provisionally accepted- 1 National University of Singapore, Singapore, Singapore
- 2 College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, Fujian Province, China
- 3 Tibet Academy of Agricultural and Animal Husbandry Sciences, Lhasa, China
Inonotus hispidus, commonly referred to as the Sanghuang mushroom, is a species that is consumed as a tea. To date, this is the only species of the same fungus that has been successfully cultivated. A single-factor test was conducted using Inonotus hispidus MS-5 and MS-9 as test materials. The response surface methodology was adopted to design and optimise the liquid fermentation medium for them. As indicated in the results, the optimum fermentation conditions for MS-5 include 24.09 g/L glucose, 7.88 g/L yeast extract, 0.99 g/L dandelion powder, 1.5 g MgSO4, 2 g KH2PO4, 0.01 g vitamin B1, and 1 L deionized water; the optimum fermentation conditions for MS-9 include 24.64 g/L glucose, 7.77 g/L yeast extract, 0.98 g/L dandelion powder, 1.5 g MgSO4, 2 g KH2PO4, 0.01 g vitamin B1, and 1 L deionized water. Under such conditions, the mycelial biomass (dry weight) values were able to reach 16.02 g/L and 14.91 g/L for MS-5 and MS-9, respectively, which were 1.6 and 1.54 times those measured before optimization. As revealed in the antioxidant and anticancer experiment, Inonotus hispidus exopolysaccharides has corresponding functional effects at the cellular level. This research optimised the liquid culture formulation of Inonotus hispidus and demonstrated that the function of it as a traditional Sanghuang herbal tea is well-documented.
Keywords: Inonotus hispidus1, exopolysaccharides (EPS)2, response surface optimization3, antioxidation4, anticancer5
Received: 28 Jun 2024; Accepted: 26 Aug 2024.
Copyright: © 2024 Gao, Li, Xu, Sun, Zhang, Wu and Fu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Junsheng Fu, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, Fujian Province, China
Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.