AUTHOR=Dalle Vedove Elena , Benvenga Alessia , Nicolai Gianluca , Massimini Marcella , Giordano Maria Veronica , Di Pierro Francesco , Bachetti Benedetta 

TITLE=Antibiotic-induced dysbiosis in the SCIME™ recapitulates microbial community diversity and metabolites modulation of in vivo disease

JOURNAL=Frontiers in Microbiology

VOLUME=15

YEAR=2024

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2024.1455839

DOI=10.3389/fmicb.2024.1455839

ISSN=1664-302X

ABSTRACT=<sec><title>Establishing the context</title><p>Intestinal dysbiosis is a significant concern among dog owners, and the gut health of pets is an emerging research field. In this context, the Simulator of the Canine Intestinal Microbial Ecosystem (SCIME™) was recently developed and validated with <italic>in vivo</italic> data.</p></sec><sec><title>Stating the purpose/introducing the study</title><p>The current study presents a further application of this model by using amoxicillin and clavulanic acid to induce dysbiosis, aiming to provoke changes in microbial community and metabolite production, which are well-known markers of the disease <italic>in vivo</italic>.</p></sec><sec><title>Describing methodology</title><p>Following the induction of dysbiosis, prebiotic supplementation was tested to investigate the potential for microbiota recovery under different dietary conditions.</p></sec><sec><title>Presenting the results</title><p>The results showed that antibiotic stimulation in the SCIME™ model can produce significant changes in microbial communities and metabolic activity, including a decrease in microbial richness, a reduction in propionic acid production, and alterations in microbial composition. Additionally, changes in ammonium and butyric acid levels induced by the tested diets were observed.</p></sec><sec><title>Discussing the findings</title><p>This alteration in microbial community and metabolites production mimicks <italic>in vivo</italic> canine dysbiosis patterns. A novel dynamic <italic>in vitro</italic> model simulating canine antibiotic-induced dysbiosis, capable of reproducing microbial and metabolic changes observed <italic>in vivo</italic>, has been developed and is suitable for testing the effects of nutritional changes.</p></sec>