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ORIGINAL RESEARCH article

Front. Microbiol.
Sec. Systems Microbiology
Volume 15 - 2024 | doi: 10.3389/fmicb.2024.1455839
This article is part of the Research Topic Omics Research in Canine and Feline Microbiome: Implications for Veterinary Medicine and Companion Animal Health View all 3 articles

Antibiotic-induced dysbiosis in the SCIME™ recapitulates microbial community diversity and metabolites modulation of in vivo disease

Provisionally accepted
Elena Dalle Vedove Elena Dalle Vedove 1*Alessia Benvenga Alessia Benvenga 1Gianluca Nicolai Gianluca Nicolai 1Marcella Massimini Marcella Massimini 2Maria Veronica Giordano Maria Veronica Giordano 3Francesco Di Pierro Francesco Di Pierro 4Benedetta Bachetti Benedetta Bachetti 1
  • 1 R&D Division, C.I.A.M. Srl, Ascoli Piceno, Italy
  • 2 Department of Veterinary Medicine, University of Teramo, Teramo, Italy
  • 3 Endovet Professional Association, Roseto degli Abruzzi, Italy
  • 4 Department of Medicine and Surgery, University of Insubria, Varese, Italy

The final, formatted version of the article will be published soon.

    Intestinal dysbiosis is a significant concern among dog owners, and the gut health of pets is an emerging research field. In this context, the Simulator of the Canine Intestinal Microbial Ecosystem (SCIME™) was recently developed and validated with in vivo data. The current study presents a further application of this model by using amoxicillin and clavulanic acid to induce dysbiosis, aiming to provoke changes in microbial community and metabolite production, which are well-known markers of the disease in vivo. Following the induction of dysbiosis, prebiotic supplementation was tested to investigate the potential for microbiota recovery under different dietary conditions. The results showed that antibiotic stimulation in the SCIME™ model can reproduce significant changes in microbial communities and metabolic activity, including a decrease in microbial richness, a reduction in propionic acid production, and alterations in microbial composition, mimicking in vivo canine dysbiosis. Additionally, changes in ammonium and butyric acid levels induced by the tested diets were observed. In conclusion, a dynamic in vitro model simulating canine antibiotic-induced dysbiosis, capable of reproducing microbial and metabolic changes observed in vivo, has been developed and is suitable for testing the effects of nutritional changes.

    Keywords: Gut disease, dog, intestinal microbiota, in vitro alternative to animal testing, Prebiotics

    Received: 27 Jun 2024; Accepted: 06 Aug 2024.

    Copyright: © 2024 Dalle Vedove, Benvenga, Nicolai, Massimini, Giordano, Di Pierro and Bachetti. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Elena Dalle Vedove, R&D Division, C.I.A.M. Srl, Ascoli Piceno, Italy

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.