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ORIGINAL RESEARCH article

Front. Microbiol.
Sec. Food Microbiology
Volume 15 - 2024 | doi: 10.3389/fmicb.2024.1455008
This article is part of the Research Topic Postharvest Management of Fresh Produce Using Beneficial Microbes View all articles

Cell-free supernatant of Bacillus tequilensis reduces grey mold caused by Botrytis cinerea of blueberry fruit: Inducing phenylpropanoid pathway metabolism

Provisionally accepted
Qianjie Du Qianjie Du *Raoyong Li Raoyong Li Li Liu Li Liu Lin Chen Lin Chen Junrong Tang Junrong Tang Jia Deng Jia Deng FANG WANG FANG WANG *
  • Southwest Forestry University, Kunming, China

The final, formatted version of the article will be published soon.

    Botrytis cinerea a blueberry grey mould, is one of the main diseases affecting postharvest storage, causing significant losses. To better control the postharvest gray mould of blueberry, the effects of KXF 6501 fermentation solution (YY) and KXF6501 cell-free supernatant (SQ) on the induction of disease resistance in blueberry fruits were studied using biochemical and transcriptomic analyses. The results showed that YY and SQ controlled the conidial germination and mycelial growth of B. cinerea in vitro. After 3 d of culture, the lesion diameter and incidence of gray mould in blueberry fruits inoculated with YY and SQ were significantly smaller than those in the control group. Transcription spectrum analysis of blueberry peel tissue showed that the YY- and SQ-induced phenylpropane metabolic pathways had more differentially expressed genes (DEGs) than other biological pathways. In addition, biochemical analyses showed that YY and SQ treatments effectively enhanced the activity of enzymes related to the phenylpropane pathway (phenylalanine ammonia-lyase [PAL], cinnamate 4-hydroxylase [C4H], 4-coumarate CoA ligase [4CL], and polyphenol oxidase [PPO]) and stimulated the synthesis of lignin, total phenols, and flavonoids. Compared with the control, the YY and SQ treatments reduced the weight loss rate and better maintained the appearance and nutritional quality of the blueberry fruits. Thus, it can be concluded that Bacillus tequilensis KXF6501 is potentially useful as a suitable bio-control agent in harvested blueberries.

    Keywords: Bacillus tequilensis, Blueberry, induced resistance, phenylpropanoids, Postharvest disease, biological control

    Received: 26 Jun 2024; Accepted: 06 Aug 2024.

    Copyright: © 2024 Du, Li, Liu, Chen, Tang, Deng and WANG. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Qianjie Du, Southwest Forestry University, Kunming, China
    FANG WANG, Southwest Forestry University, Kunming, China

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.