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ORIGINAL RESEARCH article

Front. Microbiol.
Sec. Microbial Symbioses
Volume 15 - 2024 | doi: 10.3389/fmicb.2024.1453617

Skin microbiota during metamorphosis of Quasipaa spinosa: Guidance for maintaining mucosal symbiotic microbial flora homeostasis healthy aquaculture in early life of frogs

Provisionally accepted
Jinliang Hou Jinliang Hou Yu Tan Yu Tan Yanfei Huang Yanfei Huang Hong Li Hong Li Deliang Li Deliang Li Xinhua Liu Xinhua Liu Junhua Li Junhua Li Yazhou Hu Yazhou Hu *Jianguo Xiang Jianguo Xiang *
  • Hunan Agricultural University, Changsha, China

The final, formatted version of the article will be published soon.

    The skin microbiota plays an essential role in helping the host adapt to different environments and maintain health. By examining the characteristics of amphibian skin flora alongside ontogenetic traits, we can gain insights into the adaptation mechanisms of amphibian skin flora to environmental changes during development. In this study, we analyzed the skin microbiota of Quasipaa spinosa during metamorphosis using Illumina sequencing. Venn diagrams and UpSet analysis revealed that the LTS (hindlimb tadpoles’ skin, aquatic habitat) and FTS (forelimb tadpoles’ skin, shift from aquatic to amphibious habitats) groups exhibited a higher number of unique amplicon sequence variants (ASVs), while the TS (tadpoles’ skin, aquatic habitat) and LFS (land frogs’ skin, amphibious habitats) groups displayed a lower abundance of ASVs. Diversity analysis indicated similarities in the microorganisms between the LTS and the FTS groups, with higher microbial diversity compared to the TS and the LFS groups. Additionally, microbial co-occurrence network analysis indicated a more stable microecology in the LTS group and FTS group. Proteobacteria, Firmicutes, and Bacteroidota were identified as the dominant phyla, although their relative abundances varied widely among groups. LEfSe (Linear discriminant analysis effect size) showed significant enrichment of beneficial bacteria at various developmental stages, including Bacteroides, Bacillus, and Lactobacillus. Functional prediction analysis shows significant differences in skin microorganism functions across various developmental stages, with a primary focus on metabolic functions. This study provides valuable insights into the compositional dynamics of skin microbiota in Q. spinosa at various developmental stages.

    Keywords: Developmental Stage, Quasipaa spinosa, Cutaneous bacterial communities, 16S amplicon sequencing, Co-occurrence network

    Received: 27 Jun 2024; Accepted: 04 Nov 2024.

    Copyright: © 2024 Hou, Tan, Huang, Li, Li, Liu, Li, Hu and Xiang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Yazhou Hu, Hunan Agricultural University, Changsha, China
    Jianguo Xiang, Hunan Agricultural University, Changsha, China

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.