AUTHOR=Mori Koji , Watanabe Mizuki , Nanri Keiko , Matsukura Satoko , Ota Yuri , Homma Nobuyuki , Noda Naohiro TITLE=Clear zone formation in microdroplets for high-throughput screening for lactic acid bacteria JOURNAL=Frontiers in Microbiology VOLUME=15 YEAR=2024 URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2024.1452573 DOI=10.3389/fmicb.2024.1452573 ISSN=1664-302X ABSTRACT=
Droplet microfluidic-based technology is a powerful tool for biotechnology, and it is also expected that it will be applied to culturing and screening methods. Using this technology, a new high-throughput screening method for lactic acid bacteria was developed. In this study, the conventional culture of lactic acid bacteria that form clear zones on an agar medium was reproduced in water-in-oil droplets, and only the droplets in which lactic acid bacteria grew were collected one by one. Using this method, the specific recovery of