AUTHOR=Horie Masanori , Ruengsomwong Supatjaree , Ohmiya Yoshihiro TITLE=Analysis of lactic acid bacteria species in Miang, a post-fermented tea in Thailand, and their potential use as probiotics JOURNAL=Frontiers in Microbiology VOLUME=15 YEAR=2024 URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2024.1450158 DOI=10.3389/fmicb.2024.1450158 ISSN=1664-302X ABSTRACT=Introduction

Miang is one of the post-fermented teas made in Northern Thailand. Although lactic acid bacteria are involved in fermentation of Miang, details are still not clear. This study investigated the diversity of Lactobacillaceae bacteria, related to fermentation of Miang. Probiotic potential of isolated Lactobacillaceae bacteria was examined.

Methods

Lactobacillaceae bacteria were isolated from 52 Miang samples collected from three provinces in northern Thailand and identified by MALDI-TOF MS. Hemolytic activity, antibiotic susceptibility, antimicrobial activity and tolerance to gastrointestinal juice were examined for probiotic potential of isolates.

Results

A total of 1,181 Lactobacillaceae bacteria strains were isolated from Miang. The most abundant isolates were Lactiplantibacillus pentosus. Besides Lactiplantibacillus plantarum, Levilactobacillus brevis, Paucilactobacillus suebicus, Lacticaseibacillus pantheris, and Secundilactobacillus collinoides were also found with frequency. Of these isolates, 450 with a high score for MALDI-TOF identification were then screened for probiotic ability. Most isolates were resistant to aminoglycosides and clindamycin. Then, 35 isolates were tested for their antimicrobial activity against pathogens using the well diffusion method, and 31 isolates exhibited inhibition zones against Staphylococcus aureus, Staphylococcus epidermidis, Salmonella enterica serovar Typhimurium, S. enterica serovar Enteritidis, Listeria monocytogenes, Propionibacterium acnes, and Streptococcus mutans. All 31 isolates were non-hemolytic and readily tolerated simulated gastric juice at pH 3 and simulated intestinal juice at pH 8.

Discussion

Miang contains lactic acid bacteria that could potentially be used as probiotics.