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ORIGINAL RESEARCH article

Front. Microbiol.
Sec. Terrestrial Microbiology
Volume 15 - 2024 | doi: 10.3389/fmicb.2024.1447488
This article is part of the Research Topic Soil Microbial Communities to Promote Suppressiveness against Soil-Borne Pathogens and Diseases View all articles

Biocontrol and growth promotion potential of Bacillus velezensis NT35 on Panax ginseng based on the multifunctional effect

Provisionally accepted
Xueqing Li Xueqing Li 1*Jiarui Wang Jiarui Wang 2*Hang Shen Hang Shen 1*Chenxi Xing Chenxi Xing 1*Lingxin Kong Lingxin Kong 1*Yu Song Yu Song 2*Wanpeng Hou Wanpeng Hou 3*Jie Gao Jie Gao 1*Yun Jiang Yun Jiang 2*Changqing Chen Changqing Chen 1*
  • 1 College of Plant Protection, Jilin Agriculture University, Changchun, China
  • 2 College of Life Sciences, Jilin Agricultural University, Changchun, Jilin Province, China
  • 3 Jilin Shenwang Plant Protection Co., Ltd., fusong, China

The final, formatted version of the article will be published soon.

    Abstract: The Bacillus velezensis strain NT35, which has strong biocontrol ability, was isolated from the rhizosphere soil of Panax ginseng. The antifungal effects of the NT35 strain against the mycelium and spore growth of Ilyonectria robusta, which causes ginseng rusty root rot, were determined. The inhibitory rate of I. robusta mycelial growth was 94.12% when the concentration of the NT35 strain was 107 CFU·mL-1, and the inhibitory rates of I. robusta sporulation and spore germination reached 100% and 90.31%, respectively, when the concentration of the NT35 strain was 104 CFU·mL-1 and 108 CFU·mL-1, respectively. Strain NT35 had good prevention effects against ginseng rust rot indoors and in the field with the control effect 51.99%, which was similar to that of commercial chemical and biocontrol agents. The labelled strain NT35-Rif160-Stre400 was obtained and colonized ginseng roots, leaves, stems and rhizosphere soil after 90 days. B. velezensis NT35 can induce a significant increase in the expression of five defensive enzyme-encoding genes and ginsenoside biosynthesis-related genes in ginseng. In the rhizosphere soil, the four soil enzymes and the microbial community improved during different periods of ginseng growth in response to the biocontrol strain NT35. The NT35 strain can recruit several beneficial bacteria, such as Luteimonas, Nocardioides, Sphingomonas, and Gemmatimonas, from the rhizosphere soil and reduce the relative abundance of Ilyonectria, Fusarium, Neonectria and Dactylonectria, which cause root rot and rusty root rot in ginseng plants. The disease indices were significantly negatively correlated with the abundances of Sphingomonas and Trichoderma. Additionally, Sphingomonadales, Sphingomonadaceae and actinomycetes were significantly enriched under the NT35 treatment according to LEfSe analysis. These results lay the foundation for the development of a biological agent based on strain NT35.

    Keywords: Bacillus velezensis, Panax ginseng, biocontrol, plant growth promotion, multifunctional mechanisms

    Received: 11 Jun 2024; Accepted: 10 Jul 2024.

    Copyright: © 2024 Li, Wang, Shen, Xing, Kong, Song, Hou, Gao, Jiang and Chen. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Xueqing Li, College of Plant Protection, Jilin Agriculture University, Changchun, China
    Jiarui Wang, College of Life Sciences, Jilin Agricultural University, Changchun, 130118, Jilin Province, China
    Hang Shen, College of Plant Protection, Jilin Agriculture University, Changchun, China
    Chenxi Xing, College of Plant Protection, Jilin Agriculture University, Changchun, China
    Lingxin Kong, College of Plant Protection, Jilin Agriculture University, Changchun, China
    Yu Song, College of Life Sciences, Jilin Agricultural University, Changchun, 130118, Jilin Province, China
    Wanpeng Hou, Jilin Shenwang Plant Protection Co., Ltd., fusong, China
    Jie Gao, College of Plant Protection, Jilin Agriculture University, Changchun, China
    Yun Jiang, College of Life Sciences, Jilin Agricultural University, Changchun, 130118, Jilin Province, China
    Changqing Chen, College of Plant Protection, Jilin Agriculture University, Changchun, China

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