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ORIGINAL RESEARCH article

Front. Microbiol.
Sec. Microbe and Virus Interactions with Plants
Volume 15 - 2024 | doi: 10.3389/fmicb.2024.1446583

Aromatic components and endophytic fungi during the formation of agarwood in Aquilaria sinensis were induced by exogenous substances

Provisionally accepted
Shengjiang Pang Shengjiang Pang 1,2*Weiwei Zhao Weiwei Zhao 3Qingqing Zhang Qingqing Zhang 4*Zuwei Tian Zuwei Tian 1*Dan Wu Dan Wu 5*Shuokun Deng Shuokun Deng 1*Pei Zhang Pei Zhang 1*Zhongguo Li Zhongguo Li 1*Shiling Liu Shiling Liu 1*Baoguo Yang Baoguo Yang 1*Guihua Huang Guihua Huang 3*Zaizhi Zhou Zaizhi Zhou 3*
  • 1 Experimental Center of Tropical Forestry, Chinese Academy of Forestry, Pingxiang, China
  • 2 Nanjing Forestry University, Nanjing, Jiangsu Province, China
  • 3 Research Institute of Tropical Forestry, Chinese Academy of Forestry, Guangzhou, Guangdong Province, China
  • 4 Fujian Academy of Forestry, Fuzhou, Fujian Province, China
  • 5 Guangxi International Zhuang Medical Hospital, Nanning, Guangxi Zhuang Region, China

The final, formatted version of the article will be published soon.

    The process of formation of aromatic components for agarwood in Aquilaria sinensis is closely related to endophytic fungi and the result of complex multiple long-term joint interactions with them.However, the interactions between the aromatic components and endophytic fungi remain unclear during the formation of agarwood. In this study, precise mixed solution of hormones, inorganic salts, and fungi was used to induce its formation in A. sinensis, and sample blocks of wood were collected at different times after inoculation. This study showed that the aromatic compounds found in the three treatments of A. sinensis were primarily chromones (31.70%-33.65%), terpenes (16.68%-27.10%), alkanes (15.99%-23.83%), and aromatics (3.13%-5.07%). Chromones and terpenes were the primary components that characterized the aroma. The different sampling times had a more pronounced impact on the richness and diversity of endophytic fungal communities in the A. sinensis xylem than the induction treatments. The species annotation of the operational taxonomic units (OTUs) demonstrated that the endophytic fungi were primarily composed of 18 dominant families and 20 dominant genera. A linear regression analysis of the network topology properties with induction time showed that the interactions among the fungal species continued to strengthen, and the network structure tended to become more complex. The terpenes significantly negatively correlated with the Pielou evenness index (P< 0.05), while the chromones significantly positively correlated with the OTUs and Shannon indices.

    Keywords: Aquilaria sinensis, Aromatic components, Endophytic fungal diversity, agarwood-induction technique, Induction time, Agarwood

    Received: 10 Jun 2024; Accepted: 06 Aug 2024.

    Copyright: © 2024 Pang, Zhao, Zhang, Tian, Wu, Deng, Zhang, Li, Liu, Yang, Huang and Zhou. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Shengjiang Pang, Experimental Center of Tropical Forestry, Chinese Academy of Forestry, Pingxiang, China
    Qingqing Zhang, Fujian Academy of Forestry, Fuzhou, 350012, Fujian Province, China
    Zuwei Tian, Experimental Center of Tropical Forestry, Chinese Academy of Forestry, Pingxiang, China
    Dan Wu, Guangxi International Zhuang Medical Hospital, Nanning, Guangxi Zhuang Region, China
    Shuokun Deng, Experimental Center of Tropical Forestry, Chinese Academy of Forestry, Pingxiang, China
    Pei Zhang, Experimental Center of Tropical Forestry, Chinese Academy of Forestry, Pingxiang, China
    Zhongguo Li, Experimental Center of Tropical Forestry, Chinese Academy of Forestry, Pingxiang, China
    Shiling Liu, Experimental Center of Tropical Forestry, Chinese Academy of Forestry, Pingxiang, China
    Baoguo Yang, Experimental Center of Tropical Forestry, Chinese Academy of Forestry, Pingxiang, China
    Guihua Huang, Research Institute of Tropical Forestry, Chinese Academy of Forestry, Guangzhou, Guangdong Province, China
    Zaizhi Zhou, Research Institute of Tropical Forestry, Chinese Academy of Forestry, Guangzhou, Guangdong Province, China

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