Skip to main content

ORIGINAL RESEARCH article

Front. Microbiol.
Sec. Microbial Physiology and Metabolism
Volume 15 - 2024 | doi: 10.3389/fmicb.2024.1444996

Antifungal Metabolites of Biocontrol Stain LB-1 and Its Inhibition Mechanism Against Botrytis cinerea

Provisionally accepted
Huan Zhang Huan Zhang Hongliang Ji Hongliang Ji *Caiyun Liu Caiyun Liu *
  • Weifang University, Weifang, China

The final, formatted version of the article will be published soon.

    Chaetomium subaffine LB-1 is a novel biocontrol strain which produces non-volatile metabolites that inhibit the growth of Botrytis cinerea. However, the specific metabolites and antimicrobial mechanism of the strain LB-1 remains unclear. In this study, the antifungal substances produced by stain LB-1 as well as the underlying mechanism of its inhibitory effect against B. cinerea were explored using metabolomic and transcriptomic analysis. Results found that 45 metabolites might be the key antifungal substance such as ouabain, ferulic acid, chlorogenic acid, spermidine, stachydrine, and stearic acid. The transcriptomic analysis indicated that the inhibition effect of LB-1 on B. cinerea resulted in up-regulation of genes related to ABC transporters, peroxisome, ER stress, and multiple metabolic pathways, and in down-regulation of many genes associated with the synthesis of cell walls / membranes, carbohydrate metabolism, cell cycle, meiosis, and DNA replication. These results suggested that the inhibitory effect of strain LB-1 against B. cinerea might be due to the destroyed cell wall and membrane integrity exerted by antimicrobial substances, which affect cell metabolism, and inhibits cell proliferation.

    Keywords: Chaetomium subaffine, Antifungal substance, Cellular structure, cell metabolism, proliferation

    Received: 06 Jun 2024; Accepted: 12 Aug 2024.

    Copyright: © 2024 Zhang, Ji and Liu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Hongliang Ji, Weifang University, Weifang, China
    Caiyun Liu, Weifang University, Weifang, China

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.