Skip to main content

ORIGINAL RESEARCH article

Front. Microbiol.
Sec. Antimicrobials, Resistance and Chemotherapy
Volume 15 - 2024 | doi: 10.3389/fmicb.2024.1441525

Paradendryphiella arenariae an endophytic fungus of Centella asiatica inhibits the bacterial pathogens of fish and shellfish

Provisionally accepted
Saranya Shankar Saranya Shankar Mythili Sathiavelu Mythili Sathiavelu *
  • School of Biosciences and Technology, Vellore Institute of Technology, Vellore, Tamil Nadu, India

The final, formatted version of the article will be published soon.

    Aquaculture has been considered a major food-producing sector in the world during the last few decades. The foremost constraint in the development of aquaculture is bacterial disease control and management. Since various fish pathogens are resistant to conservative treatments, it is essential to screen new and effective alternative antibacterial agents.Endophytic fungi are microorganisms that live in the plant's internal tissues without harming its host. Endophytic fungi have proven themselves as reliable sources of novel bioactive compounds that can be used as antibacterial agents. The objective of the present study was to isolate, screen, and identify the endophytic fungi isolates from the stem of the Centella asiatica plant and to study the hostile efficiency against fish and shellfish pathogens. Agar plug diffusion and agar well diffusion method revealed that endophytic fungus CAS1 exhibits maximum antagonistic activity against bacterial fish pathogens. The ethyl acetate crude extract of CAS1 displayed exhibited the maximum zone of inhibitions against Aeromonas hydrophila (21 ± 0.11mm), Aeromonas caviae (18 ± 0.1mm), Edwardsiella tarda (23 ± 0.11mm), Vibrio anguillarum (19 ± 0.05mm) and Vibrio harveyi (20 ± 0.27mm). The MIC and MBC values extract varied reliant on the trial pathogens ranging between 12.5-100 µg/mL and 25-100 µg/mL correspondingly. The morphological and molecular characterization of potential isolate CAS1 was confirmed as Paradendryphiella arenariae by 18S rRNA ITS gene sequencing with 99.18% identity. This is the foremost findings to study the antagonistic effect of Paradendryphiella arenariae isolated from the stem of Centella asiatica against bacterial fish pathogens.Aquaculture, endophytic fungus Endophytic fungus, paradendryphiella arenariae Paradendryphiella arenariae, bacterial fish pathogens, centella asiatica Centella asiatica, disease control, antibacterial agents.

    Keywords: Aquaculture, Endophytic fungus, Paradendryphiella arenariae, Bacterial fish pathogens, Centella asiatica, Disease control, Antibacterial Agents

    Received: 22 Jul 2024; Accepted: 21 Oct 2024.

    Copyright: © 2024 Shankar and Sathiavelu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Mythili Sathiavelu, School of Biosciences and Technology, Vellore Institute of Technology, Vellore, 632014, Tamil Nadu, India

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.