Skip to main content

ORIGINAL RESEARCH article

Front. Microbiol.
Sec. Food Microbiology
Volume 15 - 2024 | doi: 10.3389/fmicb.2024.1437803

Expression of stress responsive genes enables Limosilactobacillus reuteri to cross-protection against acid, bile salt and freeze-drying

Provisionally accepted
Zhenzhen Liu Zhenzhen Liu 1*Zhao Xiao Zhao Xiao 2Hina I. Bangash Hina I. Bangash 2
  • 1 Chengdu University, Chengdu, China
  • 2 Huazhong Agricultural University, Wuhan, Hubei Province, China

The final, formatted version of the article will be published soon.

    Limosilactobacillus reuteri effectively colonizing the gut, secretes antimicrobial compounds and strengthens immune system function. Considering these health benefits, increasing its stress assessments efficiency could improve its commercial viability. For this purpose, the resistance of L. reuteri FP41 to acid, bile salts, and freeze-drying was examined. The findings showed that strain FP41 demonstrated a strong resistance to acid/bile salt stresses. The transcriptome revealed a significant upregulation of various stress response genes, including those related to membrane integrity, glutamine metabolism, OsmC family protein, ABC transporters, and chaperonin. Subsequent research demonstrated that overexpression of three stress response-specific proteins, including glutamate decarboxylase GatD, osmotically induced bacterial protein OsmC, and membrane protein component CsbD, significantly increased the survival rate of L. reuteri Z204 under acid/bile salts stress.Notably, overexpression of the OsmC, CsbD, and GatD proteins also enhanced the survival of L. reuteri after freeze-drying. The development of a unique crossprotection method is highlighted in this study, that might significantly increase cellular resistance to acid, bile salts, and cold stresses. This finding could significantly impact the way that L. reuteri is employed in industrial manufacturing processes.

    Keywords: Limosilactobacillus reuteri, acid stress, bile salts stress, Freeze-drying, cross-protection

    Received: 24 May 2024; Accepted: 18 Sep 2024.

    Copyright: © 2024 Liu, Xiao and Bangash. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Zhenzhen Liu, Chengdu University, Chengdu, China

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.