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ORIGINAL RESEARCH article

Front. Microbiol.
Sec. Microbe and Virus Interactions with Plants
Volume 15 - 2024 | doi: 10.3389/fmicb.2024.1437799

Green control for inhibiting Rhizopus oryzae growth by stress factors in forage grass factory

Provisionally accepted
Dedong Kong Dedong Kong *Mengdi Dai Mengdi Dai Xiangfeng Tan Xiangfeng Tan Xuting Chen Xuting Chen Kangfeng Cai Kangfeng Cai Yuanxiang Zhong Yuanxiang Zhong Ziran Ye Ziran Ye
  • Zhejiang Academy of Agricultural Sciences, Hangzhou, China

The final, formatted version of the article will be published soon.

    The forage grass factory could break through the restrictions of land resources, region and climate to achieve efficient production throughout the year by accurate and intelligent management. However, due to its closed environment, mold outbreaks in the forage grass factory were severe, significantly affecting barley production. In this study, 9 contaminated barley tissues were collected and 45 strains were isolated in forage grass factory. After ITS sequencing, 45 strains were all identified as Rhizopus oryzae. Through stress factor assays, R. oryzae growth was seriously hindered by low concentration of sodium nitrate, high pH value and ozone water treatment. High pH and ozone water affected growth mainly by altering membrane integrity of R. oryzae. Sodium nitrate inhibited the growth of R. oryzae mainly by affecting the amount of sporulation. Low concentration of sodium nitrate and ozone water did not affect the growth of barley. High concentrations of sodium nitrate (100 mM) and pH values (8-8.5) inhibited barley growth. Among them, ozone water had the most obvious inhibition effect on R. oryzae. Large-scale ozone water treatment in the forage grass factory had also played a role in restoring barley production. Taken together, the green techonology to control mold disease and maintain the safety of forage through different physicochemical methods was selected, which was of considerable application value in animal husbandry.

    Keywords: Barley1, Rhizopus oryzae2, forage grass factory3, stress factor4, green control5

    Received: 27 May 2024; Accepted: 22 Jul 2024.

    Copyright: © 2024 Kong, Dai, Tan, Chen, Cai, Zhong and Ye. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Dedong Kong, Zhejiang Academy of Agricultural Sciences, Hangzhou, China

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.