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ORIGINAL RESEARCH article
Front. Microbiol.
Sec. Food Microbiology
Volume 15 - 2024 |
doi: 10.3389/fmicb.2024.1437660
Characterization and transmission of plasmid-mediated multidrug resistance in foodborne Vibrio parahaemolyticus
Provisionally accepted
Haibo Zhou
1*
Zhaoxin Lu
1
Xinmei Liu
2*
Xiaomei Bie
1*
Xinping Cui
1*
Zuwei Wang
1*
Xiaojie Sun
2*
Jun Yang
2*- 1 Nanjing Agricultural University, Nanjing, China
- 2 Nanjing Institute for Food and Drug Control, Nanjing, Liaoning Province, China
Horizontal transmission of antimicrobial resistance genes (ARGs) mediated by mobile genetic elements is considered to be an important reason for the rapid development of resistance in Vibrio parahaemolyticus. Whole genome sequencing revealed that the multidrug-resistant V. parahaemolyticus strain NJIFDCVp52 contained two circular chromosomes and two circular plasmids (pVP52-1 and pVP52-2). Plasmid typing indicated that pVP52-1 belonged to the incompatibility group IncA/C2 and the sequence type pST3. pVP52-1 carried 12 different ARGs, an IS110-composite transposon consisting of aac(6')-Ib-cr, qnrVC1, aac(6')-Ib, dfrA14 and the IS26-mphA-IS6100 unit flanked by inverted sequences of IS5075 and IS4321. pVP52-2 carried no ARGs.A plasmid elimination assay showed that only pVP52-1 and its ARGs were lost, the loss of resistance to several antimicrobials, causing a change from the ampicillin-ampicillin/sulbactam-cefazolincefoxitin-ceftazidime-cefotaxime-imipenem-trimethoprim/ sulfamethoxazole resistance pattern to the ampicillin resistance pattern. In accordance, a conjugation transfer assay showed that only pVP52-1 and its ARGs were horizontally transferred, leading to increased antimicrobial resistance in Escherichia coli strain EC600, causing a change from the ampicillin-nalidixic acid resistance pattern to the ampicillin-ampicillin/sulbactam-cefazolin-cefoxitin-ceftazidime-cefotaxime-imipenemnalidixic acid-chloramphenicol-tetracycline-trimethoprim/sulfamethoxazole-azithromycin resistance pattern. Further transferability experiments revealed that pVP52-1 could be transferred to other enterobacterial strains of E. coli and Salmonella. Our findings provide more detailed information about ARG profiles and their transmission characteristics, suggesting the potential risk of the spread of multidrug resistance.
Keywords: Vibrio parahaemolyticus, multidrug resistance, whole genome sequencing, Plasmid, antimicrobial resistance genes, Horizontal transfer
Received: 24 May 2024; Accepted: 19 Jul 2024.
Copyright: © 2024 Zhou, Lu, Liu, Bie, Cui, Wang, Sun and Yang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Haibo Zhou, Nanjing Agricultural University, Nanjing, China
Xinmei Liu, Nanjing Institute for Food and Drug Control, Nanjing, 211198, Liaoning Province, China
Xiaomei Bie, Nanjing Agricultural University, Nanjing, China
Xinping Cui, Nanjing Agricultural University, Nanjing, China
Zuwei Wang, Nanjing Agricultural University, Nanjing, China
Xiaojie Sun, Nanjing Institute for Food and Drug Control, Nanjing, 211198, Liaoning Province, China
Jun Yang, Nanjing Institute for Food and Drug Control, Nanjing, 211198, Liaoning Province, China
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