AUTHOR=Mpeyako Lenshina A. , Hart Adam J. , Bailey Nicholas P. , Carlton Jane M. , Henrissat Bernard , Sullivan Steven A. , Hirt Robert P. 

TITLE=Comparative genomics between Trichomonas tenax and Trichomonas vaginalis: CAZymes and candidate virulence factors

JOURNAL=Frontiers in Microbiology

VOLUME=15

YEAR=2024

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2024.1437572

DOI=10.3389/fmicb.2024.1437572

ISSN=1664-302X

ABSTRACT=<sec><title>Introduction</title><p>The oral trichomonad <italic>Trichomonas tenax</italic> is increasingly appreciated as a likely contributor to periodontitis, a chronic inflammatory disease induced by dysbiotic microbiota, in humans and domestic animals and is strongly associated with its worst prognosis. Our current understanding of the molecular basis of <italic>T. tenax</italic> interactions with host cells and the microbiota of the oral cavity are still rather limited. One laboratory strain of T. tenax (Hs-4:NIH/ATCC 30207) can be grown axenically and two draft genome assemblies have been published for that strain, although the structural and functional annotation of these genomes is not available.</p></sec><sec><title>Methods</title><p>GenSAS and Galaxy were used to annotate two publicly available draft genomes for <italic>T. tenax</italic>, with a focus on protein-coding genes. A custom pipeline was used to annotate the CAZymes for <italic>T. tenax</italic> and the human sexually transmitted parasite <italic>Trichomonas vaginalis</italic>, the most well-characterized trichomonad. A combination of bioinformatics analyses was used to screen for homologs of <italic>T. vaginalis</italic> virulence and colonization factors within the <italic>T. tenax</italic> annotated proteins.</p></sec><sec><title>Results</title><p>Our annotation of the two <italic>T. tenax</italic> draft genome sequences and their comparison with <italic>T. vaginalis</italic> proteins provide evidence for several candidate virulence factors. These include candidate surface proteins, secreted proteins and enzymes mediating potential interactions with host cells and/or members of the oral microbiota. The CAZymes annotation identified a broad range of glycoside hydrolase (GH) families, with the majority of these being shared between the two <italic>Trichomonas</italic> species.</p></sec><sec><title>Discussion</title><p>The presence of candidate <italic>T. tenax</italic> virulence genes supports the hypothesis that this species is associated with periodontitis through direct and indirect mechanisms. Notably, several GH proteins could represent potential new virulence factors for both Trichomonas species. These data support a model where <italic>T. tenax</italic> interactions with host cells and members of the oral microbiota could synergistically contribute to the damaging inflammation characteristic of periodontitis, supporting a causal link between <italic>T. tenax</italic> and periodontitis.</p></sec>