Skip to main content

ORIGINAL RESEARCH article

Front. Microbiol.
Sec. Food Microbiology
Volume 15 - 2024 | doi: 10.3389/fmicb.2024.1436378
This article is part of the Research Topic Omics Technologies and Bioinformatic Tools in Probiotic Research View all 3 articles

Complete genome sequence and anti-obesity potential of Lactiplantibacillus plantarum HOM2217 in 3T3-L1 cells and high-fat diet-fed rats

Provisionally accepted
Tingting Wang Tingting Wang 1Xiao Zhang Xiao Zhang 1Linlin Fan Linlin Fan 1Ying Zhao Ying Zhao 1Zhengwen Zhang Zhengwen Zhang 1Zhonghua Cao Zhonghua Cao 1Ying Xu Ying Xu 2Suwon Lee Suwon Lee 2Chongyoon Lim Chongyoon Lim 2Shiqi Zhang Shiqi Zhang 2*
  • 1 Beijing Hanmi pharmaceutical Co., Ltd. Beijing,China, Beijing, China
  • 2 Food & Biotech R&D Center, Coree Beijing Co., Ltd., Beijing 101312, China, Beijing, China

The final, formatted version of the article will be published soon.

    The global prevalence of obesity is rising year by year, which has become a public health problem worldwide. Many animal and clinical studies have shown that Lactiplantibacillus plantarum is considered an ideal probiotic and potential supplement for the treatment of obesity. In this study, we aimed to complete the genome sequence of L. plantarum HOM2217, which was isolated from human milk, and study its physiological characteristics and anti-obesity effects in 3T3-L1 cells and rats fed a high-fat diet (HFD) to determine its potential as a starter for functional food products. Wholegenome analysis demonstrated that HOM2217 contained a single circular chromosome of 3,267,529 bp with a GC content of 44.5% and one plasmid (62,350 bp) with a GC content of 38.5%. Compared to the reference strains, HOM2217 demonstrated superior tolerance to gastrointestinal conditions, higher adhesion to intestinal epithelial cell lines, potent antimicrobial activity against Enterobacter cloacae ATCC 13047, and effective cholesterol removal ability in vitro. Treatment with heat-killed HOM2217 significantly reduced lipid accumulation and intracellular triglyceride production in 3T3-L1 adipocytes. Daily treatment of HFD-fed rats with HOM2217 for 7 weeks decreased body weight, body weight gain, and body fat without changes in food intake. HOM2217 also significantly increased the serum high-density lipoprotein cholesterol (HDL-C) level, decreased the serum tumor necrosis factor (TNF-α) and increased short-chain fatty acid (SCFA) (formic acid, acetic acid, and butyric acid) levels in the cecum. Thus, HOM2217 could potentially prevent obesity in rats by inhibiting inflammatory responses and regulating lipid metabolism and SCFAs expression. Therefore, HOM2217 has potential as an alternative treatment for obesity.

    Keywords: lactiplantibacillus plantarum, Complete genome sequence, 3T3-L1 Cells, Antiobesity effect, high-fat diets

    Received: 22 May 2024; Accepted: 27 Aug 2024.

    Copyright: © 2024 Wang, Zhang, Fan, Zhao, Zhang, Cao, Xu, Lee, Lim and Zhang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Shiqi Zhang, Food & Biotech R&D Center, Coree Beijing Co., Ltd., Beijing 101312, China, Beijing, China

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.