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ORIGINAL RESEARCH article

Front. Microbiol.
Sec. Aquatic Microbiology
Volume 15 - 2024 | doi: 10.3389/fmicb.2024.1436264

Carbon and energy metabolism for the mixotrophic culture of Chlorella vulgaris using sodium acetate as a carbon source

Provisionally accepted
Xi Yan Xi Yan 1Shengzhou Shan Shengzhou Shan 1Xiaohui Li Xiaohui Li 1Qingshan Xu Qingshan Xu 2Xiaojun Yan Xiaojun Yan 1Roger Ruan Roger Ruan 3*Pengfei Cheng Pengfei Cheng 1*
  • 1 Ningbo University, Ningbo, China
  • 2 Lijiang Cheng Hai Bao Er Biological Development Co., Ltd, Lijiang, China
  • 3 University of Minnesota Twin Cities, St. Paul, Minnesota, United States

The final, formatted version of the article will be published soon.

    There has been an emergence of a diversity of microalgal mixotrophic synergistic mechanisms due to substrate differences. In this study, the effects of the mixotrophic culture of Chlorella vulgaris were examined. The maximum values of cell density, specific growth rate, and cell dry weight of Chlorella vulgaris were 3.52*10 7 cells/mL, 0.75 d -1 , and 3.48 g/L in the mixotrophic mode, respectively. These were higher than the corresponding values of photoautotrophic or heterotrophic modes. Moreover, it was found that the concentrations of sodium bicarbonate consumed by the Chlorella vulgaris under mixotrophic and photoautotrophic modes were 635 mg/L/d and 505 mg/L/d, respectively; the concentrations of sodium acetate consumed by the Chlorella vulgaris under mixotrophic and heterotrophic modes were 614 mg/L/d and 645 mg/L/d, respectively. The activity of Rubisco was 9.36 U/mL in the mixotrophic culture, which was 3.09 and 4.85 times higher than that of the photoautotrophic and heterotrophic modes, respectively. This indicated that the differences for the carbon source absorption efficiency of Chlorella vulgaris in the mixotrophy led to different internal metabolic efficiencies when compared to photoautotroph or heterotrophy. Additionally, Chlorella vulgaris exhibits a more rapid energy metabolism efficiency when operating in the mixotrophic mode.

    Keywords: Chlorella vulgaris, Mixotrophic culture, Sodium Acetate, Carbon and energy metabolism, metabolic network

    Received: 22 May 2024; Accepted: 09 Oct 2024.

    Copyright: © 2024 Yan, Shan, Li, Xu, Yan, Ruan and Cheng. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Roger Ruan, University of Minnesota Twin Cities, St. Paul, 55108, Minnesota, United States
    Pengfei Cheng, Ningbo University, Ningbo, China

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