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ORIGINAL RESEARCH article

Front. Microbiol.
Sec. Antimicrobials, Resistance and Chemotherapy
Volume 15 - 2024 | doi: 10.3389/fmicb.2024.1430954
This article is part of the Research Topic Antibiotics in Dental Medicine: Clinical and Molecular Implications View all 6 articles

Effect of Glycerol on Properties of Chitosan/Chlorhexidine Membranes and Antibacterial Activity against Streptococcus mutans

Provisionally accepted
José A. Hachity-Ortega José A. Hachity-Ortega 1Alberto V. Jerezano-Dominguez Alberto V. Jerezano-Dominguez 2*Laura A. Pazos-Rojas Laura A. Pazos-Rojas 2,3*Abigailt Flores-Ledesma Abigailt Flores-Ledesma 2Diana d. Pazos-Guarneros Diana d. Pazos-Guarneros 2Eric Reyes-Cervantes Eric Reyes-Cervantes 4Ismael Juárez-Díaz Ismael Juárez-Díaz 2Manuel E. Medina-Lopez Manuel E. Medina-Lopez 5Mayra González-Martínez Mayra González-Martínez 2Karla A. Parra-Solar Karla A. Parra-Solar 2Brenda E. Castillo-Silva Brenda E. Castillo-Silva 2Beatriz X. Ávila-Curiel Beatriz X. Ávila-Curiel 1Jesus Hernandez-Juárez Jesus Hernandez-Juárez 6América Rivera-Urbalejo América Rivera-Urbalejo 2,7Paola Gordillo-Guerra Paola Gordillo-Guerra 8Miguel Angel Casillas Santana Miguel Angel Casillas Santana 2
  • 1 Facultad de Odontología, Universidad Autónoma Benito Juárez de Oaxaca, Oaxaca, Oaxaca, Mexico
  • 2 Facultad de Estomatología, Benemérita Universidad Autónoma de Puebla, Puebla, Mexico
  • 3 Tecnológico de Monterrey, Escuela de ingeniería y Ciencias, Puebla, Mexico
  • 4 Dirección de Innovación y Transferencia de Conocimiento, Benemérita Universidad Autónoma de Puebla, Puebla, Mexico
  • 5 Centro de Investigación en Micología Aplicada, Universidad Veracruzana, Xalapa, Veracruz, Mexico
  • 6 Centro Interdisciplinario de Investigación para el Desarrollo Integral Regional, Unidad Oaxaca, Oaxaca, Mexico
  • 7 Survival of Microorganism, LEMM, CICM, IC, Benemérita Universidad Autónoma de Puebla, Puebla, Mexico
  • 8 Departamento de Sistemas Biológicos, División de Ciencias Biológicas y de la Salud, Universidad Autónoma Metropolitana, México City, Mexico

The final, formatted version of the article will be published soon.

    Chitosan membranes with glycerol can function as an effective dispersing agent for different antibiotics or active ingredients that can be used in the treatment of diseases present in the oral cavity. The effects of the addition of glycerol on the mechanical, water absorption, swelling, pH, thickness, disintegration, rugosity, and antibacterial properties of chitosan-chlorhexidine-glycerol membranes were investigated in this study. Mechanical results indicated that chitosan membranes' rugosity, strength, flexion, and thickness differed at loading 1, 3, 5, 10, 15, and 20 % of glycerol (p < 0.05). The chitosan membranes' rugosity, dissolution, strength, and pH results were significantly enhanced by the presence of glycerol at 3, 5, and 10% concentrations. In this investigation, the antimicrobial activity model used was the inhibition of Streptococcus mutans CDBB-B-1455 by chitosan-chlorhexidine membranes. It was observed that there was no change in inhibition with different concentrations of glycerol. The results suggest that chitosan-glycerol-chlorhexidine membranes may be a potential candidate for topical antiseptic application in buccal-dental disorders caused by S. mutans, such as caries, periodontal diseases, and oral squamous cell carcinoma, helping to prevent the development of serious conditions that can compromise human health.

    Keywords: chitosan1, glycerol2, chlorhexidine3, membrane4, antibacterial5, S. mutans6

    Received: 10 May 2024; Accepted: 18 Jul 2024.

    Copyright: © 2024 Hachity-Ortega, Jerezano-Dominguez, Pazos-Rojas, Flores-Ledesma, Pazos-Guarneros, Reyes-Cervantes, Juárez-Díaz, Medina-Lopez, González-Martínez, Parra-Solar, Castillo-Silva, Ávila-Curiel, Hernandez-Juárez, Rivera-Urbalejo, Gordillo-Guerra and Casillas Santana. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Alberto V. Jerezano-Dominguez, Facultad de Estomatología, Benemérita Universidad Autónoma de Puebla, Puebla, Mexico
    Laura A. Pazos-Rojas, Facultad de Estomatología, Benemérita Universidad Autónoma de Puebla, Puebla, Mexico

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.