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ORIGINAL RESEARCH article

Front. Microbiol.
Sec. Virology
Volume 15 - 2024 | doi: 10.3389/fmicb.2024.1430396
This article is part of the Research Topic Neurotropic Virus Replication and Spread Within and Between Neurons View all 6 articles

Transcriptomic insights into pseudorabies virus suppressed cell death pathways in neuroblastoma cells

Provisionally accepted
Shinuo Cao Shinuo Cao Li Zhang Li Zhang Mo Zhou Mo Zhou *Shanyuan Zhu Shanyuan Zhu *
  • Provincial Key Laboratory of Veterinary Bio-pharmaceutical High tech Research, Jiangsu Agri-animal Husbandry Vocational College, Taizhou, China

The final, formatted version of the article will be published soon.

    Pseudorabies virus (PRV) exhibits a complex interplay of hostpathogen interactions, primarily by modulating host cell death pathways to optimize its replication and spread in Neuro-2a cells. Using high-throughput RNA sequencing, we identified 2382 upregulated differentially expressed genes (DEGs) and 3998 downregulated DEGs, indicating a intricate interaction between viral pathogenesis and host cellular responses. This research offers valuable insights into the molecular processes involved in PRV infection, highlighting the substantial inhibition of crucial cell death pathways in Neuro-2a cells, including necroptosis, pyroptosis, autophagy, ferroptosis, and cuproptosis. Cells infected with PRV exhibit decreased expression of genes critical in these pathways, potentially as a mechanism to avoid host immune reactions and ensure cell survival to support ongoing viral replication. This extensive inhibition of apoptosis and metabolic alterations highlights the sophisticated tactics utilized by PRV, enhancing our comprehension of herpesvirus biology and the feasibility of creating specific antiviral treatments. This research contributes to our understanding of how viruses manipulate host cell death and presents potential opportunities for therapeutic interventions to disrupt the virus's lifecycle.

    Keywords: pseudorabies virus, Neuro-2a cells, Transcriptomics, Inflammatory, Cell Death

    Received: 09 May 2024; Accepted: 07 Aug 2024.

    Copyright: © 2024 Cao, Zhang, Zhou and Zhu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Mo Zhou, Provincial Key Laboratory of Veterinary Bio-pharmaceutical High tech Research, Jiangsu Agri-animal Husbandry Vocational College, Taizhou, China
    Shanyuan Zhu, Provincial Key Laboratory of Veterinary Bio-pharmaceutical High tech Research, Jiangsu Agri-animal Husbandry Vocational College, Taizhou, China

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