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ORIGINAL RESEARCH article

Front. Microbiol.
Sec. Virology
Volume 15 - 2024 | doi: 10.3389/fmicb.2024.1430052
This article is part of the Research Topic Molecular Pathogenesis of Enteroviruses: Insights into Viral-Host Interactions, Pathogenic Mechanisms, and Microbiome Dynamics View all 3 articles

EV71 infection alters the lipid composition of human rhabdomyosarcoma (RD) cells derived-extracellular vesicles

Provisionally accepted
Lingxiang Mao Lingxiang Mao 1*Qing Gao Qing Gao 1Chenxuan Bao Chenxuan Bao 1Huayuan Xiang Huayuan Xiang 1Qiaoqiao Chen Qiaoqiao Chen 1,2Qianqian Gao Qianqian Gao 1Yuxuan Shen Yuxuan Shen 2Feng Huang Feng Huang 1Wenyuan He Wenyuan He 1Jianjun Wang Jianjun Wang 1
  • 1 Affiliated Kunshan Hospital of Jiangsu University, Kunshan, China
  • 2 Department of Laboratory Medicine, Affiliated People’s Hospital Jiangsu University, Zhenjiang, China

The final, formatted version of the article will be published soon.

    Previous studies demonstrated that EV71-infected cells secrete extracellular vesicles (EVs), facilitating the transfer of viral components to recipient cells and thereby promoting virus spread. Considering lipid signaling plays a crucial role in EVs-mediated cell-to-cell communication, we compared the lipid profile of EVs secreted from uninfected and EV71-infected cells (EVs-Mock and EVs-EV71) using the human rhabdomyosarcoma (RD) cell model. These two groups of EVs were purified by using size exclusion chromatography (SEC) respectively and evaluated by transmission electron microscopy (TEM), nanoparticle tracking technology (NTA), and Western blotting (WB). In-depth lipidomic analysis of EVs identified 1705 lipid molecules belonging to 43 lipid classes. The data showed a significant increase in the lipid content of EVs after EV71 infection. Meanwhile, we deeply analyzed the changes in lipids and screened for lipid molecules with significant differences compared EVs-EV71 with EVs-Mock EVs. Altogether, we report the alterations in the lipid profile of EVs derived from RD-cells after EV71 infection, which may affect the function of the EVs in the recipient cells.

    Keywords: lipidomics, Lipid, extracellular vesicles, EV71, Sec

    Received: 09 May 2024; Accepted: 26 Jun 2024.

    Copyright: © 2024 Mao, Gao, Bao, Xiang, Chen, Gao, Shen, Huang, He and Wang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Lingxiang Mao, Affiliated Kunshan Hospital of Jiangsu University, Kunshan, China

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