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ORIGINAL RESEARCH article

Front. Microbiol.
Sec. Microbe and Virus Interactions with Plants
Volume 15 - 2024 | doi: 10.3389/fmicb.2024.1429646

Evaluation of Streptomyces sporoverrucosus B-1662strains for biological control of red pepper anthracnose and apple bitter rot diseases in Korea

Provisionally accepted
Dayoung Kim Dayoung Kim 1Younmi Lee Younmi Lee 1Kotnala Balaraju Kotnala Balaraju 2Ye-Ji Hwang Ye-Ji Hwang 3Mi-hwa Lee Mi-hwa Lee 3,4Wonsu Cheon Wonsu Cheon 3Hye Yeon Mun Hye Yeon Mun 3Chang Soo Lee Chang Soo Lee 3Yongho Jeon Yongho Jeon 5*
  • 1 Department of Plant Medicals, Andong National University, Andong 36729, Republic of Korea, Andong, North Gyeongsang, Republic of Korea
  • 2 Agricultural Science & Technology Research Institute, Andong National University, Andong 36729, Korea, Andong, North Gyeongsang, Republic of Korea
  • 3 Nakdonggang National Institute of Biological Resources, Sangju, North Gyeongsang, Republic of Korea
  • 4 Nakdonggang National Institute of Biological Resources, Sangju, Republic of Korea
  • 5 Department of Plant Medicine, College of Life Sciences and Health Welfare, Andong National University, Andong, Republic of Korea

The final, formatted version of the article will be published soon.

    Fungi are the prominent phytopathogens that have significant impact on the productivity of agriculture worldwide. Streptomyces species have been extensively studied for the production of various bioactive metabolites. These metabolites have been used as biocontrol agents for the management of diseases caused by phytopathogenic fungi. The purpose of this investigation is to assess the efficacy of Streptomyces sporoverrucosus B-1662, an antagonistic agent in the control of red pepper anthracnose caused by Colletotrichum acutatum KACC 42403 and apple anthracnose caused by Colletotrichum siamense CGCP6 (GYUN-10348). On the basis of the morphological, biochemical and molecular characterization using 16S rRNA, the strain B-1662 was determined to be S. treptomyces sporoverrucosus. The strain B-1662 exhibited antagonistic activity against seven fungal phytopathogens, including C. acutatum KACC 42403 and C. siamense CGCP6. The culture filtrates (CF) from B-1662 showed antifungal activity against all seven fungal pathogens with greater inhibition rate (%) in comparison with a control. The bacterial suspensions of B-1662 showed an excellent biological control effect on the red pepper anthracnose and apple bitter rot using an in planta assay. The anthracnose disease rate (%) was controlled by 서식 지정함: 강조 100%, with B-1662 cell suspensions at 10 5 and 10 7 CFU/mL, and the same case was observed in apple bitter rot with B-1662 cell suspensions at 10 6 CFU/mL The anthracnose disease rate (%) was controlled by over 90% with B-1662 cell suspensions at 10 5 to 10 7 CFU/mL. Compared to a control, the strain B-1662 played a more effective role in controlling the anthracnose disease in field conditions in both years 2022 and 2023. From the effective solvent fractions, the effect compound (dibutoxybutane) has been isolated exhibiting with antifungal effect. The genetic mechanismbase underlying the biocontrol traits of B-1662 was characterized using the whole-genome sequence of B-1662, which was compared with closely related strains. Consequently, these results collectively suggest that S. sporoverrucosus B-1662 can aid in the management of red-pepper anthracnose.

    Keywords: Colletotrichum, Capsicum annum, Biological control agent, Antimicrobial activity, secondary metabolites, Streptomyces, apple, Red pepper

    Received: 08 May 2024; Accepted: 16 Oct 2024.

    Copyright: © 2024 Kim, Lee, Balaraju, Hwang, Lee, Cheon, Mun, Lee and Jeon. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Yongho Jeon, Department of Plant Medicine, College of Life Sciences and Health Welfare, Andong National University, Andong, Republic of Korea

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