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ORIGINAL RESEARCH article

Front. Microbiol.
Sec. Food Microbiology
Volume 15 - 2024 | doi: 10.3389/fmicb.2024.1429213
This article is part of the Research Topic Insights in Food Microbiology: 2023/24 View all 5 articles

Identification of Bacterial Communities Associated with Needle Mushroom (Flammulina filiformis) and its Production Environment

Provisionally accepted
Zhenghui Liu Zhenghui Liu 1Yunlong Cong Yunlong Cong 2Frederick Leo Sossah Frederick Leo Sossah 3Hongyan Sheng Hongyan Sheng 4Yu Li Yu Li 5*
  • 1 Southwest Biomedical Resources of the Ministry of Education, Guizhou University, Guiyang, People’s Republic of China, Guiyang, Guizhou, China
  • 2 Research Institute of Science and Technology, Guizhou University, Guiyang 550025, China, Guiyang, Guizhou Province, China
  • 3 Council for Scientific and Industrial Research (CSIR), Oil Palm Research Institute, Coconut Research Programme, Sekondi P.O. Box 245, Ghana, Sekondi, Ghana
  • 4 Department of Plant Pathology, Washington State University, Pullman, WA 99164-6430, USA, Pullman, Washington, United States
  • 5 Engineering Research Center of Edible and Medicinal Fungi, Ministry of Education, Jilin Agricultural University, Changchun 130118, China, Changchun, Hebei Province, China

The final, formatted version of the article will be published soon.

    Flammulina filiformis is an important edible and medicinal mushroom widely cultivated in East Asia.Its quality and health are strongly influenced by associated microbial communities. However, data on these bacterial communities associated with F. filiformis cultivation in Chinese farms are limited.This study investigated the bacterial communities in F. filiformis and its production environment using high-throughput 16S rRNA gene amplicon sequencing and culture-dependent methods. A total of 42 samples were collected from farms located in Jilin and Guizhou provinces. The analysis revealed diverse bacterial phyla including Proteobacteria, Firmicutes, Bacteroidetes, Actinobacteria, and Cyanobacteria. Genera such as Pseudomonas, Lactobacillus, Acinetobacter, Flavobacterium, and Phyllobacterium were identified. The relative abundance of Pseudomonas and Lactobacillus varied Identification of Bacterial Communities Associated with Needle Mushroom (Flammulina filiformis) and its Production Environment between the provinces. Phenotypic, biochemical, and molecular analyses identified pathogenic species, including Pseudomonas tolaasii, Ewingella americana, Stenotrophomonas maltophilia, Pseudomonas sp., Lelliottia amnigena, and Janthinobacterium lividum. Pathogenicity tests confirmed the disease-causing potential of P. tolaasii, E. americana, and J. lividum in F. filiformis. These findings highlight regional differences in bacterial community composition. They emphasize the need for tailored management practices. This study supports safe, high-quality mushroom and offers insights for improved cultivation practices, including Mushroom Good Agricultural Practices (MGAP).

    Keywords: Flammulina filiformis, Cultivation environment, 16S amplicon, bacterial diversity, Cultivable bacteria, pathogenicity determination

    Received: 07 May 2024; Accepted: 25 Nov 2024.

    Copyright: © 2024 Liu, Cong, Sossah, Sheng and Li. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Yu Li, Engineering Research Center of Edible and Medicinal Fungi, Ministry of Education, Jilin Agricultural University, Changchun 130118, China, Changchun, Hebei Province, China

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.