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ORIGINAL RESEARCH article

Front. Microbiol.
Sec. Antimicrobials, Resistance and Chemotherapy
Volume 15 - 2024 | doi: 10.3389/fmicb.2024.1429035

Exploring the Antimicrobial and Antioxidant Properties of Lentzea flaviverrucosa strain E25-2 Isolated from Moroccan Forest Soil

Provisionally accepted
Said Rammali Said Rammali Ciobica Alin Ciobica Alin Mohamed El aalaoui Mohamed El aalaoui Abdellatif Rahim Abdellatif Rahim Fatima Zahra Kamal Fatima Zahra Kamal Khadija Dari Khadija Dari *Abdelkrim KHATTAB Abdelkrim KHATTAB *Laura Romila Laura Romila *Bogdan Novac Bogdan Novac *Antoneta Dacia Petroaie Antoneta Dacia Petroaie *Bouchaib Bencharki Bouchaib Bencharki *
  • Alexandru Ioan Cuza University, Iași, Romania

The final, formatted version of the article will be published soon.

    The alarming rise in antimicrobial resistance (AMR) has created a significant public health challenge, necessitating the discovery of new therapeutic agents to combat infectious diseases and oxidative stress-related disorders. The Lentzea flaviverrucosa strain E25-2, isolated from Moroccan forest soil, represents a potential avenue for such research. This study aimed to identify the isolate E25-2, obtained from soil in a cold Moroccan ecosystem, and further investigate its antimicrobial and antioxidant activities.Phylogenetic analysis based on 16S rRNA gene sequences revealed the strain's classification within the Lentzea genus, with a sequence closely resembling that of Lentzea flaviverrucosa AS4.0578 (96.10% similarity). Antimicrobial activity in solid media showed moderate to strong activity against Staphylococcus aureus ATCC 25923, Bacillus cereus strain ATCC 14579, Escherichia coli strain ATCC 25922, Candida albicans strain ATCC 60193 and 4 phytopathogenic fungi. In addition, ethyl acetate extract of this isolate demonstrated potent antimicrobial activity against 7 clinically multidrug resistant bacteria. Furthermore, it demonstrated antioxidant activity against 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) free radicals, as well as a significant increase in ferric reducing antioxidant power. A significant positive correlation was observed between antioxidant activities and total content of phenolic compounds (P < 0.0001), along with flavonoids (P < 0.0001).Furthermore, GC-MS analysis revealed the presence of amines, hydroxyl groups, pyridopyrazinone rings, esters and pyrrolopyrazines. The Lentzea genus could offer promising prospects in the fight against antibiotic resistance and in the prevention against oxidative stress related diseases.

    Keywords: Lentzea flaviverrucosa, Antimicrobial activity, antioxidant activity, 16S rRNA gene sequences, Hemolytic activity, GC-MS

    Received: 08 May 2024; Accepted: 05 Jul 2024.

    Copyright: © 2024 Rammali, Alin, El aalaoui, Rahim, Kamal, Dari, KHATTAB, Romila, Novac, Petroaie and Bencharki. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Khadija Dari, Alexandru Ioan Cuza University, Iași, Romania
    Abdelkrim KHATTAB, Alexandru Ioan Cuza University, Iași, Romania
    Laura Romila, Alexandru Ioan Cuza University, Iași, Romania
    Bogdan Novac, Alexandru Ioan Cuza University, Iași, Romania
    Antoneta Dacia Petroaie, Alexandru Ioan Cuza University, Iași, Romania
    Bouchaib Bencharki, Alexandru Ioan Cuza University, Iași, Romania

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.