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ORIGINAL RESEARCH article
Front. Microbiol.
Sec. Infectious Agents and Disease
Volume 15 - 2024 |
doi: 10.3389/fmicb.2024.1422574
Rapid and sensitive detection of methicillin-resistant Staphylococcus aureus through the RPA-PfAgo system
Provisionally accepted- 1 Chaozhou People's Hospital, Chaozhou, Guangdong, China
- 2 Chaozhou Central Hospital, Chaozhou, Guangdong Province, China
- 3 College of Medicine, Shantou University, Shantou, Guangdong Province, China
- 4 Youjiang Medical University for Nationalities, Baise, Guangx, China
- 5 Huizhou Central People's Hospital, Huizhou, Guangdong Province, China
Both the incidence and mortality rates associated with methicillin-resistant Staphylococcus aureus (MRSA) have progressively increased worldwide. A nucleic acid testing system was developed in response, enabling swift and precise detection of Staphylococcus aureus (S. aureus) and its MRSA infection status. This facilitates improved prevention and control of MRSA infections. In this work, we introduce a novel assay platform developed by integrating Pyrococcus furiosus Argonaute (PfAgo) with recombinase polymerase amplification (RPA), which was designed for the simultaneous detection of the nuc and mecA genes in MRSA. This innovative approach enables visual MRSA detection within 55 minutes, boasting a detection limit of 10 2 copies/μL. Characterized by its high specificity, the platform accurately identifies MRSA infections without cross-reactivity to other clinical pathogens, highlighting its unique capability for S. aureus infection diagnostics amidst bacterial diversity.Validation of this method was performed on 40 clinical isolates, demonstrating a 95.0% accuracy rate in comparison to the established Vitek2-COMPACT system. The RPA-PfAgo platform has emerged as a superior diagnostic tool, offering enhanced sensitivity, specificity, and identification efficacy for MRSA detection. Our findings underscore the potential of this platform to significantly improve the diagnosis and management of MRSA infection.
Keywords: PfAgo, RPA, POCT, Nucleic acid detection, Staphylococcus aureus, Methicillin-Resistant Staphylococcus aureus
Received: 25 Apr 2024; Accepted: 05 Aug 2024.
Copyright: © 2024 Chen, Zhang, Huagui, Su, L, Liang, Chen, Zhou, Li, Lu and Sun. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Jiexiu Zhang, College of Medicine, Shantou University, Shantou, 515041, Guangdong Province, China
Jie Su, Chaozhou Central Hospital, Chaozhou, Guangdong Province, China
Jie L, Chaozhou People's Hospital, Chaozhou, Guangdong, China
Xueyan Liang, Huizhou Central People's Hospital, Huizhou, 516001, Guangdong Province, China
Jiangtao Chen, Huizhou Central People's Hospital, Huizhou, 516001, Guangdong Province, China
Rong Zhou, Chaozhou People's Hospital, Chaozhou, Guangdong, China
Lin Li, Chaozhou People's Hospital, Chaozhou, Guangdong, China
Zefang Lu, Chaozhou People's Hospital, Chaozhou, Guangdong, China
Guangyu Sun, Chaozhou People's Hospital, Chaozhou, Guangdong, China
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