AUTHOR=Sulieman Abdel Moneim Elhadi , Abdallah Emad M. , Alanazi Naimah Asid , Idriss Hajo , Adnan Mohd , Jamal Arshad , Shommo Sohair A. M. , Snoussi Mejdi 

TITLE=Bioactive profiling of Rumex vesicarius L. from the Hail region of Saudi Arabia: a study on its phytochemical and elemental analysis, antibiofilm, antibacterial, antioxidant properties, and molecular docking analysis

JOURNAL=Frontiers in Microbiology

VOLUME=15

YEAR=2024

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2024.1421143

DOI=10.3389/fmicb.2024.1421143

ISSN=1664-302X

ABSTRACT=<sec><title>Background</title><p><italic>Rumex vesicarius</italic> is a wild leafy plant belonging to the family Polygonaceae, renowned for its therapeutic benefits. The genus Rumex comprises approximately 150 species distributed globally.</p></sec><sec><title>Objective</title><p>The study aimed to investigate the biological activities of <italic>R. vesicarius</italic> using in vitro and <italic>in silico</italic> methods.</p></sec><sec><title>Methods</title><p><italic>Rumex vesicarius</italic> was collected from the mountains in Hail and extracted with methanol. The phytochemical composition was qualitatively determined using colorimetric detection methods. Additional analyses included elemental analysis, <italic>in silico</italic> docking, antioxidant, antibacterial, and anti-biofilm properties.</p></sec><sec><title>Results</title><p>The extract contained various classes of phytochemicals, including flavonoids, phenolics, tannins, terpenes, and saponins. Sixteen constituents were identified through molecular docking, revealing inhibition against the filamentous temperature-sensitive protein Z (FtsZ), a crucial factor in bacterial cell division. Six compounds exhibited low binding scores ranging from −8.3 to −5.0 kcal/mol, indicating efficient interaction at the active site. Elemental analysis identified 15 elements, with potassium being the most abundant, followed by calcium, aluminum, silicon, iron, phosphorus, sulfur, magnesium, titanium, strontium, zinc, manganese, bromine, and chromium. Antioxidant analysis revealed significant properties at lower concentrations compared to ascorbic acid, butylated hydroxytoluene, and β-carotene. Antibacterial analysis demonstrated inhibitory effects on Bacillus subtilis MTCC121 and <italic>Pseudomonas aeruginosa</italic> MTCC 741, with inhibition zones of 13.67 ± 1.0 mm and 11.50 ± 1.0 mm, respectively. The MIC and MBC values ranged from 250 to 500 μg/mL. <italic>R. vesicarius</italic> also exhibited anti-biofilm activity.</p></sec><sec><title>Conclusion</title><p>Wild-grown <italic>R. vesicarius</italic> from the mountains of Hail is rich in bioactive phytochemicals and essential minerals, exhibiting notable antioxidant and antibacterial properties.</p></sec>