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ORIGINAL RESEARCH article

Front. Microbiol.
Sec. Microorganisms in Vertebrate Digestive Systems
Volume 15 - 2024 | doi: 10.3389/fmicb.2024.1420103
This article is part of the Research Topic Rodent model organisms: Human diets and the gut microbiome View all 22 articles

Alterations of the gut microbiota and metabolites by ShenZhu TiaoPi granule improves hyperglycaemia in GK rats

Provisionally accepted
Jindong Zhao Jindong Zhao *Zhaohui Fang Zhaohui Fang *
  • First Affiliated Hospital of Anhui University of Traditional Chinese Medicine, Hefei, China

The final, formatted version of the article will be published soon.

    ShenZhu TiaoPi granule (STG) is a compound prescription of Chinese medicine applied for the treatment of type 2 diabetes mellitus (T2DM). Previous studies have indicated a hypoglycaemic effect, but the underlying mechanism remains unclear. GK rats were used to establish an in vivo T2DM model (Mod). The metformin (Met) and STG treatment time was 12 weeks. Fasting blood glucose (FBG) and insulin levels and the area under the glucose curve (GAUC) were measured. Intestinal pathology and permeability were observed. Microbial diversity and metabolomics were used to investigate the underlying mechanisms. Compared with the Con group, the T2DM Mod group presented significant differences in weight, FBG, GAUC, and HOMA-IR indices (P < 0.01). Met and STG improved these indicators (P < 0.01). The pathological morphology and ZO-1 protein levels in the intestines of the Mod group of rats were altered, leading to increases in the LPS and IL-1β levels. These intestinal conditions improved, and LPS and IL-1β levels were significantly decreased in the Met and STG groups (P < 0.01). Changes in the gut microbiota and metabolites occurred in the model group. In the STG groups, the abundance of Intestinimonas increased, and the abundance of Eubacterium coprostanoligenes decreased significantly (P<0.05). Moreover, STG also regulated 2-deoxyglucose, beta-muricholic acid and dioxolithocholic acid production. In addition, the main metabolic pathways affected by STG were bile acid biosynthesis and cholesterol metabolism. Intestinimonas and D-Maltose_and_Alpha-Lactose may be potential biomarkers for the effects of STG. STG improves hyperglycaemia via the gut microbiota and metabolites in GK rats.

    Keywords: type 2 diabetes mellitus, improving hyperglycaemia, Gut Microbiota, gut metabolites, ShenZhu TiaoPi Granule

    Received: 22 Apr 2024; Accepted: 29 Jul 2024.

    Copyright: © 2024 Zhao and Fang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Jindong Zhao, First Affiliated Hospital of Anhui University of Traditional Chinese Medicine, Hefei, China
    Zhaohui Fang, First Affiliated Hospital of Anhui University of Traditional Chinese Medicine, Hefei, China

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