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ORIGINAL RESEARCH article

Front. Microbiol.
Sec. Microbiotechnology
Volume 15 - 2024 | doi: 10.3389/fmicb.2024.1419461
This article is part of the Research Topic Navigating Challenges and Innovations in Antimicrobial Resistance, Environmental Microbiology, and Industrial Solutions View all articles

Dimethylformamide degradation in Paracoccus sulfuroxidans DM175A1-1 isolated from activated sludge of wastewater

Provisionally accepted
Gang Zheng Gang Zheng 1,2Yue Su Yue Su 3Wenwu Zhang Wenwu Zhang 3Xingping Liu Xingping Liu 4Li Shao Li Shao 4Zhibo Shen Zhibo Shen 5Dongdong Zhang Dongdong Zhang 1Kaiyang Wang Kaiyang Wang 6Zhoudi Miao Zhoudi Miao 6*
  • 1 Ocean College, Zhejiang University, Zhoushan, China
  • 2 Xianghu Laboratory, Hangzhou, China
  • 3 Other, Hangzhou, China
  • 4 Zhoushan Sewage Treatment Co., Ltd., Zhoushan, China
  • 5 Other, Zhoushan, China
  • 6 Ocean Research Center of Zhoushan, Zhejiang University, Zhoushan, China

The final, formatted version of the article will be published soon.

    N,N-dimethylformamide (DMF) is an organic solvent with stable chemical properties and high boiling point. Based on its good solubility, DMF is widely used in synthetic textile, leather, electronics, pharmaceutical and pesticide industries. However, the DMF pollutes the environment and does harm to human liver function, kidney function, and nerve function. Herein, an efficient DMF-degrading strain, DM175A1-1, was isolated and identified as Paracoccus sulfuroxidans. This strain can use DMF as the sole source of carbon and nitrogen. Whole-genome sequencing of strain DM175A1-1 revealed that it has a 3.99-Mbp chromosome a 120-kbp plasmid1 and a 40-kbp plasmid2. The chromosome specifically harbors the dmfA1 and dmfA2 essential for the initial steps of DMF degradation. And it also carries the some part of genes facilitating subsequent methylotrophic metabolism and glutathione-dependent pathway. Through further DMF tolerance degradation experiments, DM175A1-1 can tolerate DMF concentrations up to 10000 mg/L, whereas the majority of Paracoccus strains could only show degradation activity below 1000 mg/L. And the efficiency of organic nitrogen conversion to NH3-N in DMF can reach 99.0% when the hydraulic retention time (HRT) is controlled at 5 d. Meanwhile, it showed a significant degradation effect at a pharmaceutical enterprise in Zhejiang Province with high concentration of DMF wastewater. This study provides a new strain Paracoccus sulfuroxidans DM175A1-1 which shows a significant influence on DMF degradation, and reveals the characterization on its DMF degradation. It lays the foundation for the application of biological method in the efficient degradation of DMF in industrial wastewater.

    Keywords: N, N-dimethylformamide, Paracoccus sulfuroxidans, High tolerance, Efficient degradation, application

    Received: 03 May 2024; Accepted: 09 Aug 2024.

    Copyright: © 2024 Zheng, Su, Zhang, Liu, Shao, Shen, Zhang, Wang and Miao. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Zhoudi Miao, Ocean Research Center of Zhoushan, Zhejiang University, Zhoushan, China

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.