AUTHOR=Zhou Ding-Xi , Kong Xiang-Ming , Huang Xiong-Min , Li Na , Feng Na , Xu Jun-Wei 

TITLE=Breeding a new Ganoderma lucidum strain with increased contents of individual ganoderic acids by mono–mono crossing of genetically modified monokaryons

JOURNAL=Frontiers in Microbiology

VOLUME=15

YEAR=2024

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2024.1410368

DOI=10.3389/fmicb.2024.1410368

ISSN=1664-302X

ABSTRACT=<p>Ganoderic acids (GAs) are major functional components of <italic>Ganoderma lucidum</italic>. The study aimed to breed a new <italic>G. lucidum</italic> strain with increased contents of individual GAs. Two mating-compatible monokaryotic strains, G. 260125 and G. 260124, were successfully isolated from the dikaryotic <italic>G. lucidum</italic> CGMCC 5.0026 via protoplast formation and regeneration. The <italic>Vitreoscilla</italic> hemoglobin gene (<italic>vgb</italic>) and squalene synthase gene (<italic>sqs</italic>) were overexpressed in the monokaryotic G. 260124 and G. 260125 strain, respectively. Mating between the G. 260124 strain overexpressing <italic>vgb</italic> and the G. 260125 strain overexpressing sqs resulted in the formation of the new hybrid dikaryotic <italic>G. lucidum</italic> strain sqs-vgb. The maximum contents of ganoderic acid (GA)-T, GA-Me, and GA-P in the fruiting body of the mated sqs-vgb strain were 23.1, 15.3, and 39.8 μg/g dry weight (DW), respectively, 2.23-, 1.75-, and 2.69-fold greater than those in <italic>G. lucidum</italic> 5.0026. The squalene and lanosterol contents increased 2.35- and 1.75-fold, respectively, in the fruiting body of the mated sqs-vgb strain compared with those in the <italic>G. lucidum</italic> 5.0026. In addition, the maximum expression levels of the <italic>sqs</italic> and lanosterol synthase gene (<italic>ls</italic>) were increased 3.23- and 2.13-fold, respectively, in the mated sqs-vgb strain. In summary, we developed a new <italic>G. lucidum</italic> strain with higher contents of individual GAs in the fruiting body by integrating genetic engineering and mono–mono crossing.</p>