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ORIGINAL RESEARCH article

Front. Microbiol.
Sec. Infectious Agents and Disease
Volume 15 - 2024 | doi: 10.3389/fmicb.2024.1409597

The impact of oxygen content on Staphylococcus epidermidis pathogenesis in ocular infection based on clinical characteristics, transcriptome and metabolome analysis

Provisionally accepted
Hongling Lv Hongling Lv Wenjia Zhang Wenjia Zhang *Zhu Zhao Zhu Zhao *Zhengyilin Bao Zhengyilin Bao *Yingpu Wei Yingpu Wei *Yizheng Li Yizheng Li *Zhulin Hu Zhulin Hu *Deyao Deng Deyao Deng *Wenli Yuan Wenli Yuan *
  • The Affiliated Hospital of Yunnan University, Kunming, Yunnan Province, China

The final, formatted version of the article will be published soon.

    This study aims to delineate the etiology and prevalence of isolated pathogens, along with the clinical characteristics of endophthalmitis patients over a 9-year period at hospital in Southwest of China. Additionally, we investigating the metabolic and cellular processes related to environmental factors may offer novel insights into endophthalmitis. For this, we analyzed data pertaining to endophthalmitis patients treated at the Affiliated Hospital of Yunnan University from 2015 to 2023. According to our clinical data, we conducted an experiment based on transcriptomics and metabolomics analysis to verify whether environmental factors affect behavior of S. epidermidis by culturating S. epidermidis under oxic and microoxic condition. In this study, 2712 fungi or bateria strains have been analyzed, gram-positive bacteria constituted 65.08%, with S. epidermidis being the most predominant species (25.55%). Ophthalmic trauma was the primary pathogenic factor for S. epidermidis ocular infections. Regarding fluoroquinolones, S. epidermidis exhibited the higher resistance rate to levofloxacin than moxifloxacin. Moreover, our investigation revealed that S. epidermidis in microoxic environment increase in energy metabolism, 字体颜色: 红色 设置格式[Administrator]: (51.4%) 删除[Administrator]: 删除[Administrator]: (15.8%) 删除[Administrator]:amino acid metabolism, and membrane transport. Our findings underscore the significance of S. epidermidis as a crucial pathogen responsible for infectious endophthalmitis. It is crucial to exercise vigilance when considering Levofloxacin as the first-line drug for empiric endophthalmitis treatment. The metabolites alteration observed during the commensal-to-pathogen conversion under microoxic condition serve as a pivotal environmental signal contributing to S. epidermidis metabolism remodeling, toward more pathogenic state.

    Keywords: S. epidermidis, Ocular infection, transcriptomic, Metabolomics, oxygen content

    Received: 30 Mar 2024; Accepted: 01 Jul 2024.

    Copyright: © 2024 Lv, Zhang, Zhao, Bao, Wei, Li, Hu, Deng and Yuan. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Wenjia Zhang, The Affiliated Hospital of Yunnan University, Kunming, 650106, Yunnan Province, China
    Zhu Zhao, The Affiliated Hospital of Yunnan University, Kunming, 650106, Yunnan Province, China
    Zhengyilin Bao, The Affiliated Hospital of Yunnan University, Kunming, 650106, Yunnan Province, China
    Yingpu Wei, The Affiliated Hospital of Yunnan University, Kunming, 650106, Yunnan Province, China
    Yizheng Li, The Affiliated Hospital of Yunnan University, Kunming, 650106, Yunnan Province, China
    Zhulin Hu, The Affiliated Hospital of Yunnan University, Kunming, 650106, Yunnan Province, China
    Deyao Deng, The Affiliated Hospital of Yunnan University, Kunming, 650106, Yunnan Province, China
    Wenli Yuan, The Affiliated Hospital of Yunnan University, Kunming, 650106, Yunnan Province, China

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