AUTHOR=Rey-Velasco Xavier , Lucena Teresa , Belda Ana , Gasol Josep M. , Sánchez Olga , Arahal David R. , Pujalte María J. 

TITLE=Genomic and phenotypic characterization of 26 novel marine bacterial strains with relevant biogeochemical roles and widespread presence across the global ocean

JOURNAL=Frontiers in Microbiology

VOLUME=15

YEAR=2024

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2024.1407904

DOI=10.3389/fmicb.2024.1407904

ISSN=1664-302X

ABSTRACT=<p>Prokaryotes dominate global oceans and shape biogeochemical cycles, yet most taxa remain uncultured and uncharacterized as of today. Here we present the characterization of 26 novel marine bacterial strains from a large isolate collection obtained from Blanes Bay (NW Mediterranean) microcosm experiments made in the four seasons. Morphological, cultural, biochemical, physiological, nutritional, genomic, and phylogenomic analyses were used to characterize and phylogenetically place the novel isolates. The strains represent 23 novel bacterial species and six novel genera: three novel species pertaining to class <italic>Alphaproteobacteria</italic> (families <italic>Rhodobacteraceae</italic> and <italic>Sphingomonadaceae</italic>), six novel species and three new genera from class <italic>Gammaproteobacteria</italic> (families <italic>Algiphilaceae</italic>, <italic>Salinispheraceae</italic>, and <italic>Alteromonadaceae</italic>), 13 novel species and three novel genera from class <italic>Bacteroidia</italic> (family <italic>Flavobacteriaceae</italic>), and one new species from class <italic>Rhodothermia</italic> (family <italic>Rubricoccaceae</italic>). The bacteria described here have potentially relevant roles in the cycles of carbon (e.g., carbon fixation or energy production via proteorhodopsin), nitrogen (e.g., denitrification or use of urea), sulfur (oxidation of sulfur compounds), phosphorus (acquisition and use of different forms of phosphorus and remodeling of membrane phospholipids), and hydrogen (oxidation of hydrogen to obtain energy). We mapped the genomes of the presented strains to the Tara Oceans metagenomes to reveal that these strains were globally distributed, with those of the family <italic>Flavobacteriaceae</italic> being the most widespread and abundant, while <italic>Rhodothermia</italic> being the rarest and most localized. While molecular-only approaches are also important, our study stresses the importance of culturing as a powerful tool to further understand the functioning of marine bacterial communities.</p>