AUTHOR=Intrator Naomi , Jayakumar Amal , Ward Bess B. 

TITLE=Aquatic nitrous oxide reductase gene (nosZ) phylogeny and environmental distribution

JOURNAL=Frontiers in Microbiology

VOLUME=15

YEAR=2024

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2024.1407573

DOI=10.3389/fmicb.2024.1407573

ISSN=1664-302X

ABSTRACT=<p>Nitrous oxide (N<sub>2</sub>O) is a potent greenhouse gas and a major cause of ozone depletion. One-third of atmospheric N<sub>2</sub>O originates in aquatic environments. Reduction of N<sub>2</sub>O to dinitrogen gas (N<sub>2</sub>) requires the nitrous oxide reductase enzyme, which is encoded by the gene <italic>nosZ</italic>. Organisms that contain <italic>nosZ</italic> are the only known biological sinks of N<sub>2</sub>O and are found in diverse genera and a wide range of environments. The two clades of <italic>nosZ</italic> (Clade I and II) contain great diversity, making it challenging to study the population structure and distribution of <italic>nosZ</italic> containing organisms in the environment. A database of over 11,000 <italic>nosZ</italic> sequences was compiled from NCBI (representing diverse aquatic environments) and unpublished sequences and metagenomes (primarily from oxygen minimum zones, OMZs, where N<sub>2</sub>O levels are often elevated). Sequences were clustered into archetypes based on DNA and amino acid sequence identity and their clade, phylogeny, and environmental source were determined. Further analysis of the source and environmental distribution of the sequences showed strong habitat separation between clades and phylogeny. Although there are more Clade I <italic>nosZ</italic> genes in the compilation, Clade II is more diverse phylogenetically and has a wider distribution across environmental sources. On the other hand, Clade I <italic>nosZ</italic> genes are predominately found within marine sediment and are primarily from the phylum Pseudonomonadota. The majority of the sequences analyzed from marine OMZs represented distinct phylotypes between different OMZs showing that the <italic>nosZ</italic> gene displays regional and environmental separation. This study expands the known diversity of <italic>nosZ</italic> genes and provides a clearer picture of how the clades and phylogeny of <italic>nosZ</italic> organisms are distributed across diverse environments.</p>