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ORIGINAL RESEARCH article

Front. Microbiol.
Sec. Food Microbiology
Volume 15 - 2024 | doi: 10.3389/fmicb.2024.1407270
This article is part of the Research Topic Postharvest Management of Fresh Produce Using Beneficial Microbes View all articles

Functional characterization and genomic analysis of Bacillus megaterium with the ability to degrade aflatoxin B1

Provisionally accepted
Ting Li Ting Li Xiaoxi Chang Xiaoxi Chang Zixuan Qiao Zixuan Qiao Guangxi Ren Guangxi Ren Na Zhou Na Zhou Jiaxin Chen Jiaxin Chen Dan Jiang Dan Jiang *Liu Chunsheng Liu Chunsheng *
  • Beijing University of Chinese Medicine, Beijing, China

The final, formatted version of the article will be published soon.

    Coix seed is a good product for both medicinal and food use, which is highly susceptible to Aflatoxin B1(AFB1) contamination during field transport, storage and processing. The aim of this study is to find microbial strains that can solve the problem of contamination of Coix seed. In this study, the AFB1degrading microorganism SX1-1 was isolated and identified as a Bacillus megaterium based on morphology, microscopy and 16S rDNA sequencing. The optimum culture conditions for SX1-1 to degrade AFB1 were determined to be 12 h. The optimum degradation conditions were 72 h, 57°C and an initial pH of 8.0. The active component of this strain was the fermentation supernatant, which was able to degrade 97.45% of AFB1 in liquid medium. In addition, whole-genome sequencing analysis of this strain revealed the presence of a number of enzymes that could potentially degrade AFB1. Importantly, SX1-1 was able to degrade AFB1contaminated Coix seeds in situ by 50.06% after co-culture. In conclusion, this strain had a high AFB1 degradation ability, and has great potential and great application as a biocontrol agent for AFB1 degradation of Coix seed.

    Keywords: Aflatoxin B1, Bacillus megaterium, Coix seed, Fermentation supernatant, Whole-genome sequencing

    Received: 26 Mar 2024; Accepted: 02 Jul 2024.

    Copyright: © 2024 Li, Chang, Qiao, Ren, Zhou, Chen, Jiang and Chunsheng. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Dan Jiang, Beijing University of Chinese Medicine, Beijing, China
    Liu Chunsheng, Beijing University of Chinese Medicine, Beijing, China

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.