Skip to main content

REVIEW article

Front. Microbiol.
Sec. Microbiotechnology
Volume 15 - 2024 | doi: 10.3389/fmicb.2024.1406632

Advancements and Applications of Loop-mediated Isothermal Amplification (LAMP) Technology: A Comprehensive Overview

Provisionally accepted
Nan Yang Nan Yang 1Han Zhang Han Zhang 1Xiu Han Xiu Han 2Zhifeng Liu Zhifeng Liu 1*Yan Lu Yan Lu 1*
  • 1 Department of Gastroenterology, Children’s Hospital of Nanjing Medical University, Nanjing, China
  • 2 Jiangsu Key Laboratory of Experimental and Translational Non-Coding RNA Research, Medical College, Yangzhou University, Yangzhou, China

The final, formatted version of the article will be published soon.

    Loop-mediated isothermal amplification (LAMP) is a novel method for nucleic acid detection known for its isothermal properties, high efficiency, sensitivity, and specificity. LAMP employs 4 to 6 primers targeting 6 to 8 regions of the desired sequence, allowing for amplification at temperatures between 60-65℃ and the production of up to 10^9 copies within a single hour.The abundance of amplicons and by-products generated during LAMP amplification enables detection through The product can be monitored by various methods such as turbidimetry, fluorometry, and colorimetry, facilitating both real-time monitoring and endpoint analysis. However, it faces limitations such as the risk of non-specific amplification, challenges in primer design, unsuitability for short gene sequences, and difficulty in multiplexing.Recent advancements in polymerase and primer design have enhanced the speed and convenience of the LAMP reaction. Additionally, integrating LAMP with technologies like rolling circle amplification (RCA), recombinase polymerase amplification (RPA), and CRISPR-Cas systems has enhanced its efficiency. The combination of LAMP with various biosensors has enabled real-time analysis, broadening its application in point-of-care testing (POCT).Furthermore, the integration of LAMP with other technologies has broadened its potential applications.This paper provides an overview of the fundamental principles, monitoring methods, applications, and recent technical advancements in LAMP, as well as the synergistic applications of LAMP with other technologies . Microfluidic technology has further facilitated the automation and miniaturization of LAMP assays, allowing for the simultaneous detection of multiple targets and preventing contamination. This review highlights advancements in LAMP, focusing on primer design, polymerase engineering, and its integration with other technologies. Continuous improvements and integration of LAMP with complementary technologies have significantly enhanced its diagnostic capabilities, making it a robust tool for rapid, sensitive, and specific nucleic acid detection with promising implications for healthcare, agriculture, and environmental monitoring.

    Keywords: Loop-mediated isothermal amplification, Monitoring methods, DNA polymerase, Primer Design, Molecular diagnosis, Technical improvement

    Received: 25 Mar 2024; Accepted: 04 Jul 2024.

    Copyright: © 2024 Yang, Zhang, Han, Liu and Lu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Zhifeng Liu, Department of Gastroenterology, Children’s Hospital of Nanjing Medical University, Nanjing, China
    Yan Lu, Department of Gastroenterology, Children’s Hospital of Nanjing Medical University, Nanjing, China

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.