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ORIGINAL RESEARCH article

Front. Microbiol.
Sec. Antimicrobials, Resistance and Chemotherapy
Volume 15 - 2024 | doi: 10.3389/fmicb.2024.1404996
This article is part of the Research Topic Resources for Developing Plasmid Databases View all 6 articles

Emergence of bla NDM-1 -carrying Enterobacter chengduensis in China

Provisionally accepted
Hongyu Fu Hongyu Fu 1Zhichen Zhu Zhichen Zhu 1Xiao Wang Xiao Wang 2Jingnan Lv Jingnan Lv 1Jie Zhu Jie Zhu 1Liang Chen Liang Chen 3Hua Yu Hua Yu 2Hong Du Hong Du 1*
  • 1 Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu Province, China
  • 2 Sichuan Provincial People’s Hospital, Chengdu, China
  • 3 University at Buffalo, Buffalo, New York, United States

The final, formatted version of the article will be published soon.

    Enterobacter chengduensis was defined as a novel species in the genus Enterobacter in 2019, however, antimicrobial resistance, such as carbapenem resistance, has rarely been described in E. chengduensis. This study described the molecular features of four carbapenem-resistant E. chengduensis strains collected from a tertiary health care hospital in Southwest China. Whole genome sequencing (WGS) was used to determine the genome sequence of four E. chengduensis strains.The precise species of strains were identified by average nucleotide identity (ANI) and in silico DNA-DNA hybridization (isDDH). The clonal relatedness of four E. chengduensis strains and additional 15 ones from NCBI were examined through phylogenetic analysis. The molecular features of E. chengduensis and genetic structure of carbapenemase-encoding plasmids were characterized through genomic annotation and analysis. The results revealed the emergence of blaNDM-1-carrying E. chengduensis strains in China. Multilocus sequence typing (MLST) analysis showed that all 19 E. chengduensis belonged to the same sequence type of ST414. Core SNP analysis suggested the potential intrahospital clonal transmission of ST414 E. chengduensis. The carbapenemase-encoding gene blaNDM-1 was harbored by an IncCtype plasmid, which was experimentally confirmed to be able to conjugate. This study reports the first emergence and potential clonal transmission of blaNDM-1-carrying E. chengduensis. Further surveillance should be advocated to monitor the dissemination of carbapenem-resistant E. chengduensis and blaNDM-1-harboring IncC-type plasmids in China.

    Keywords: Enterobacter chengduensis, carbapenemase, BlaNDM-1, ST414, IncC

    Received: 22 Mar 2024; Accepted: 31 Jul 2024.

    Copyright: © 2024 Fu, Zhu, Wang, Lv, Zhu, Chen, Yu and Du. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Hong Du, Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu Province, China

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