AUTHOR=Tanaka Tomoki , Sugiyama Ryoga , Sato Yu , Kawaguchi Manami , Honda Kohsuke , Iwaki Hiroaki , Okano Kenji TITLE=Precise microbiome engineering using natural and synthetic bacteriophages targeting an artificial bacterial consortium JOURNAL=Frontiers in Microbiology VOLUME=15 YEAR=2024 URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2024.1403903 DOI=10.3389/fmicb.2024.1403903 ISSN=1664-302X ABSTRACT=
In natural microbiomes, microorganisms interact with each other and exhibit diverse functions. Microbiome engineering, which enables bacterial knockdown, is a promising method to elucidate the functions of targeted bacteria in microbiomes. However, few methods to selectively kill target microorganisms in the microbiome without affecting the growth of nontarget microorganisms are available. In this study, we focused on the host-specific lytic ability of virulent phages and validated their potency for precise microbiome engineering. In an artificial microbiome consisting of