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ORIGINAL RESEARCH article

Front. Microbiol.
Sec. Microbe and Virus Interactions with Plants
Volume 15 - 2024 | doi: 10.3389/fmicb.2024.1400616

Understory growth of Paris polyphylla accumulates a reservoir of plants secondary metabolites

Provisionally accepted
Xinru Yan Xinru Yan 1Dong Wang Dong Wang 1*Ao Zhang Ao Zhang 1*Jing Xia Jing Xia 1*Jinlong Jiao Jinlong Jiao 1*Murad Ghanim Murad Ghanim 2Ou Xiaokun Ou Xiaokun 3*Xiahong He Xiahong He 1*Rui Shi Rui Shi 1*
  • 1 Southwest Forestry University, Kunming, Yunnan Province, China
  • 2 Insitute of plant protection, LeZion, Israel
  • 3 Yunnan University, Kunming, Yunnan Province, China

The final, formatted version of the article will be published soon.

    Paris polyphylla is an important traditional medicinal plant of the Himalayan region. It is extensively used for the production of natural steroidal saponins and flavonoids. Although, seed dormancy of wild plant can be broken to artificially maintain and regenerate through micropropagation in the laboratory, however, success of secondary metabolites production in higher quantity and synthesis of superior plant metabolites have been very limited. In this study, we present differential metabolic profiling of Paris polyphylla plants maintained for eight years in natural as well as greenhouse conditions.Untargeted profiling of metabolites through ultra-performance liquid chromatography/mass spectrometry, followed by statistical analysis, identified secondary metabolites that were enriched in naturally occurring plant roots, compared with greenhouse plant roots. Multivariate statistical analysis revealed the differential distribution of compounds between the two groups. Overall, we identified 1182 secondary metabolites, where 116 metabolites were differentially up regulated whereas 256 metabolites were down regulated. Whereas, 810 metabolites insignificantly varied in both the growing conditions. Kyoto Encyclopedia of Genes and Genomes analysis revealed that naturally forest grown Paris plants were significantly enriched in steroidal saponin, lipids, vitamins, flavonoids and flavonols. Analysis of top-10 differentially up-regulated secondary metabolites indicated significantly enriched quantity of spirost-5-en-3,12-diol and Kaempferol synthesis pathways, which are considered as free radical scavengers inside the cell. Additionally, veratramine alkaloids were also enriched in natural conditions. Our findings indicate that the naturally maintained Paris plants are suitable for extraction of medicinally important compounds. Our study establishes the causal relationship between the metabolic composition of the roots and their natural growth condition. This study highlights the importance of environmental conditions in biosynthesis of plant secondary metabolites.

    Keywords: Paris polyphylla, Rhizome, secondary metabolites, Steroidal saponin, plant flavonoids Non Gras, Ligatures : Standard + Contextuelles

    Received: 13 Mar 2024; Accepted: 16 Aug 2024.

    Copyright: © 2024 Yan, Wang, Zhang, Xia, Jiao, Ghanim, Xiaokun, He and Shi. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Dong Wang, Southwest Forestry University, Kunming, 650224, Yunnan Province, China
    Ao Zhang, Southwest Forestry University, Kunming, 650224, Yunnan Province, China
    Jing Xia, Southwest Forestry University, Kunming, 650224, Yunnan Province, China
    Jinlong Jiao, Southwest Forestry University, Kunming, 650224, Yunnan Province, China
    Ou Xiaokun, Yunnan University, Kunming, 650500, Yunnan Province, China
    Xiahong He, Southwest Forestry University, Kunming, 650224, Yunnan Province, China
    Rui Shi, Southwest Forestry University, Kunming, 650224, Yunnan Province, China

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