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ORIGINAL RESEARCH article

Front. Microbiol.
Sec. Microbiotechnology
Volume 15 - 2024 | doi: 10.3389/fmicb.2024.1394617
This article is part of the Research Topic Microbial Prospecting and Biomaterials View all 6 articles

A new and promising C-phycocyanin-producing cyanobacterial strain, Cyanobium sp. MMK01: Practical strategy towards developing a methodology to achieve C-phycocyanin with ultra-high purity

Provisionally accepted
Kambiz Akbari Noghabi Kambiz Akbari Noghabi 1*Melika Shafiei Melika Shafiei 1Maral Shafiei Maral Shafiei 1Naeema Mohseni Sani Naeema Mohseni Sani 1Hossein Shahbani Zahiri Hossein Shahbani Zahiri 1Wangbiao S. Guo Wangbiao S. Guo 2Ali A Kermani Ali A Kermani 3Shuaiqi Guo Shuaiqi Guo 4Hojatollah Vali Hojatollah Vali 4
  • 1 National Institute for Genetic Engineering and Biotechnology (Iran), Tehran, Iran
  • 2 Yale University, New Haven, Connecticut, United States
  • 3 Department of Biochemistry, St. Jude Children's Research Hospital, Memphis, Tennessee, United States
  • 4 McGill University, Montreal, Quebec, Canada

The final, formatted version of the article will be published soon.

    Selecting a suitable cyanobacterial strain and developing easy-to-afford purification processes are two crucial aspects impacting the optimal production yield and appropriate purity of C-phycocyanin (C-PC). Cyanobium sp. MMK01, a highly efficient C-PC-producing bacterium, was identified among four cyanobacterial isolates using morphological characteristics and 16S rRNA gene sequencing. The purification process of C-PC began with ammonium sulfate precipitation, leading to a purity index (PI) of 4.04. Subsequent purification through ion exchange chromatography ultimately resulted in an ultra-highly purified form of C-PC with a significant PI of 5.82. SDS-PAGE analysis of purified C-PC showed the presence of two distinct bands, α (13 kDa) and β (15 kDa). Significantly effective at scavenging free radicals, C-PC also inhibits the viability of human lung cancer cells (Calu-6). Antibacterial, anti-inflammatory, antioxidant, and cancer-preventive compounds were detected in the MMK01 cells' methanolic extract following GC-MS analysis. The promising results indicate that Cyanobium sp. MMK01 has a great deal of potential for producing C-PC that is on par with strains found in the market, and the triedand-true two-step purification process proved to work well to achieve an ultra-highly purified form of C-PC.

    Keywords: Cyanobium sp. MMK01, C-phycocyanin, Ion-exchange chromatography, Human lung cancer cells (Calu-6), MTT assay 4

    Received: 01 Mar 2024; Accepted: 23 Oct 2024.

    Copyright: © 2024 Akbari Noghabi, Shafiei, Shafiei, Mohseni Sani, Shahbani Zahiri, Guo, Kermani, Guo and Vali. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Kambiz Akbari Noghabi, National Institute for Genetic Engineering and Biotechnology (Iran), Tehran, Iran

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