Skip to main content

ORIGINAL RESEARCH article

Front. Microbiol.
Sec. Phage Biology
Volume 15 - 2024 | doi: 10.3389/fmicb.2024.1387830
This article is part of the Research Topic Bacteriophages in Food Safety View all 3 articles

A novel lytic phage infecting MDR Salmonella enterica and its application as effective food biocontrol Authors

Provisionally accepted
Anu Bala Jaglan Anu Bala Jaglan 1,2Ravikant Verma Ravikant Verma 1*Medhavi Vashisth Medhavi Vashisth 2*Nitin Virmani Nitin Virmani 2*Bidhan Chandra Bera Bidhan Chandra Bera 2Rajesh Kumar Vaid Rajesh Kumar Vaid 2Taruna Anand Taruna Anand 2*
  • 1 Department of Zoology and Aquaculture, Chaudhary Charan Singh Haryana Agricultural University, Hisar, India, Hisar, Haryana, India
  • 2 National Research Centre on Equines (ICAR), Hisar, Haryana, India

The final, formatted version of the article will be published soon.

    Salmonella enterica is a foodborne pathogen associated with both typhoid and nontyphoid illness in humans and animals. This problem is further exacerbated by the emergence of antibiotic resistant strains of Salmonella enterica. Therefore, to meet the public health and safety there is a need of an alternative strategy to tackle antibiotic resistant bacteria. Bacteriophages or the (bacterial viruses) due to their specificity, self-dosing, and anti-biofilm activity, served as a better approach in fighting against drug resistant bacteria. In the current study a broad host range lytic phage phiSalP219 was isolated against multidrug resistant Salmonella enterica serotypes Paratyphi from pond water sample. Salmonella phage phiSalP219 showed infection ability in was able to lyse 28/30 tested strains of Salmonella enterica. Salmonella phage phiSalP219 exhibits activity in acidic environment (pH3) and high temperature (70°C). Electron microscopy and genome analysis revealed that phage phiSalP219 is the member of class Caudoviricetes. Genome of Salmonella phage phiSalP219 is 146.6kKb in size with 44.5% GC content. A total of 250 CDSORFs and 256 tRNAs were predicted in its genome. Predicted ORFs were divided into five groups based on their annotation results: (1) Nucleotide metabolism, (2) DNA replication and transcription, (3) Structural proteins, (4) Lysis protein, and (5) other proteins. Absence of lysogeny related genes in their genome indicate that Salmonella phage phiSalP219 is lytic in nature. Phage phiSalP219 was also found to be microbiologically safe (due to the absence of toxin or virulence related genes) in the control of Salmonella enterica serovar Typhimuriumtyphimurium infections in the ready to eat meat and also able to eradicate biofilm formed by the same bacterium on the borosilicate glass surface.

    Keywords: Salmonella enterica, Bacteriophage, Caudoviricetes, Broad host range, genome analysis, 12 pt, Font color: Auto, Pattern: Clear Formatted: Font: Italic Formatted: Font: Italic Formatted: Font: Italic Formatted: Font: (Default) Times New Roman

    Received: 18 Feb 2024; Accepted: 01 Jul 2024.

    Copyright: © 2024 Jaglan, Verma, Vashisth, Virmani, Bera, Vaid and Anand. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Ravikant Verma, Department of Zoology and Aquaculture, Chaudhary Charan Singh Haryana Agricultural University, Hisar, India, Hisar, Haryana, India
    Medhavi Vashisth, National Research Centre on Equines (ICAR), Hisar, Haryana, India
    Nitin Virmani, National Research Centre on Equines (ICAR), Hisar, Haryana, India
    Taruna Anand, National Research Centre on Equines (ICAR), Hisar, Haryana, India

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.