AUTHOR=Li Zeyu , Luo Ning , Zhang Wenwen , Khan Raja Asad Ali , Ling Jian , Zhao Jianlong , Yang Yuhong , Mao Zhenchuan , Xie Bingyan , Zhou Ligang , Li Yan 

TITLE=Nematicidal glycosylated resorcylic acid lactones from the fungus Pochonia chlamydosporia PC-170 and their key biosynthetic genes

JOURNAL=Frontiers in Microbiology

VOLUME=15

YEAR=2024

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2024.1385255

DOI=10.3389/fmicb.2024.1385255

ISSN=1664-302X

ABSTRACT=<p>Chemical study of the nematicidal biocontrol fungus <italic>Pochonia chlamydosporia</italic> PC-170 led to discovery of six resorcylic acid lactones (RALs), including three nematicidal glycosylated RALs, monocillin VI glycoside <bold>(1)</bold>, colletogloeolactone A <bold>(2)</bold> and monocillin II glycoside <bold>(3)</bold>, and three antibacterial non-glycosylated RALs, monocillin VI <bold>(4)</bold>, monocillin IV <bold>(5)</bold> and monocillin II <bold>(6)</bold>. The planar structure of the new compound monocillin VI glycoside <bold>(1)</bold> was elucidated using HRESIMS and NMR data, and its monosaccharide configuration was further determined through sugar hydrolysis experiment and GC–MS analysis method. Furthermore, their two biosynthetic-related PKS genes, <italic>pchE</italic> and <italic>pchI</italic>, were identified through the gene knockout experiment. The glycosylated RALs <bold>1–3</bold> exhibited nematicidal activity against <italic>Meloidogyne incognita</italic>, with LC<sub>50</sub> values of 94, 152 and 64 <italic>μ</italic>g/mL, respectively, and thus had great potential in the development of new nematicidal natural products to control <italic>M. incognita</italic> in the future.</p>