ORIGINAL RESEARCH article

Front. Microbiol.

Sec. Microbe and Virus Interactions with Plants

Volume 15 - 2024 | doi: 10.3389/fmicb.2024.1384204

This article is part of the Research TopicMicroorganisms in Sustainable and Green Agriculture: Synergistic Effect on Carbon Sequestration and Crop ProductivityView all 10 articles

Comparative analysis of the soil microbiome and carbohydrate content of Anthoxanthum nitens (Sweetgrass) and other Poaceae grass tissues and associated soils

Provisionally accepted
Marissa  KingMarissa King1Barinder  BajwaBarinder Bajwa1Naomi  HannaNaomi Hanna1Xiaohui  XingXiaohui Xing1Kristin  E LowKristin E Low1Patrick  NeubergerPatrick Neuberger1Erin  HallErin Hall1Michael  VeltriMichael Veltri2Brett  WeighillBrett Weighill3,4Leeann  KlassenLeeann Klassen1Noreen  Plain EagleNoreen Plain Eagle3William  Big BullWilliam Big Bull3Laura  S LynesLaura S Lynes5Tony  MontinaTony Montina2Philippe  J ThomasPhilippe J Thomas6Monika  A GorzelakMonika A Gorzelak1Dennis  Wade AbbottDennis Wade Abbott1,2*
  • 1Lethbridge Research and Development Centre, Agriculture and Agri-Food Canada (AAFC), Lethbridge, Alberta, Canada
  • 2Department of Chemistry and Biochemistry, Faculty of Arts and Science, University of Lethbridge, Lethbridge, Alberta, Canada
  • 3Piikani Nation, Brocket, Canada
  • 4Department of Geography, Faculty of Arts and Science, University of Lethbridge, Lethbridge, Alberta, Canada
  • 5The Resilience Institute, Canmore, Alberta, Canada
  • 6Environment and Climate Change Canada, National Wildlife Research Centre, Ottawa, Canada

The final, formatted version of the article will be published soon.

Sweetgrass (Anthoxanthum nitens) is a culturally and environmentally significant perennial grass to many Indigenous Peoples; however, little is known about the potential of Sweetgrass as a contributor to soil health, biodiversity, and climate adaptation. Here, a team of transdisciplinary experts from academia, a non-governmental organization, and a First Nation community collaborated to investigate the structural composition of the rhizomes, stems, and leaves of greenhouse-grown Sweetgrass in comparison to other Poaceae grass members found in a nearby field. The data shows that the monosaccharide composition of A. nitens was evenly distributed throughout the three tissues, and that cellulose was the predominant polysaccharide followed by glucuronarabinoxylans. There were lesser amounts of xyloglucans, mixed-linkage glucans, homogalacturonans, and rhamnogalacturonans as the hemicellulosic and pectic polysaccharides, respectively. The carbohydrate composition seen in A. nitens was consistent with the other Poaceae grasses evaluated in this study, with the exception of Setaria chondrachne, which contained elevated pectin levels in its stems and leaves. Additionally, the analysis of the carbohydrate content within the soil samples revealed a higher abundance of carbohydrates within greenhouse soil when compared to field soil samples, with significantly more mannose, galactose, and galacturonic acid. Further, there were structural differences in the microbial communities across sampling sites, including a significant Unclassified / Non classifié increase in the abundance of Bacillus spp. in the greenhouse soil. Overall, this study provides the glycome and associated soil microbial community baseline for greenhouse-grown Sweetgrass.

Keywords: Sweetgrass, Anthoxanthum nitens, soil microbiome, Glycomics, indigenous knowledge, Transdisciplinary

Received: 08 Feb 2024; Accepted: 06 May 2024.

Copyright: © 2024 King, Bajwa, Hanna, Xing, Low, Neuberger, Hall, Veltri, Weighill, Klassen, Plain Eagle, Big Bull, Lynes, Montina, Thomas, Gorzelak and Abbott. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Dennis Wade Abbott, Lethbridge Research and Development Centre, Agriculture and Agri-Food Canada (AAFC), Lethbridge, T1J 4B1, Alberta, Canada

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