AUTHOR=Sun Yanan , Yan Yanfei , Yan Shaofei , Li Fengqin , Li Ying , Yan Lin , Yang Dajin , Peng Zixin , Yang Baowei , Sun Jiali , Xu Jin , Dong Yinping , Bai Yao 

TITLE=Prevalence, antibiotic susceptibility, and genomic analysis of Vibrio alginolyticus isolated from seafood and freshwater products in China

JOURNAL=Frontiers in Microbiology

VOLUME=15

YEAR=2024

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2024.1381457

DOI=10.3389/fmicb.2024.1381457

ISSN=1664-302X

ABSTRACT=<sec><title>Introduction</title><p>This study characterized <italic>Vibrio alginolyticus</italic> isolated from seafood and freshwater products in China (2020).</p></sec><sec><title>Methods and Results</title><p>In total, 122 (95.31%) <italic>V. alginolyticus</italic> isolates were resistant to at least 1 antibiotic category, and 2 (1.56%) isolates were resistant to at least 3 antibiotic categories and belong to multi-drug resistance (MDR) isolates. A high prevalence rate was observed to be <italic>blaCARB</italic> (98.04%) encoding beta-lactam resistance, followed by <italic>tet</italic> (97.06%) encoding tetracycline resistance and <italic>fos</italic> (4.90%) encoding resistance to fosfomycin. Among the 57 <italic>V. alginolyticus</italic> isolates, the commonest virulence genes were type III secretion system translocated gene <italic>vopD</italic>, <italic>vopB</italic>, and <italic>vcrH</italic> (54.4%, 31/57), type III secretion system regulated gene <italic>tyeA</italic> (54.39%), followed by <italic>vscI</italic> and <italic>vscF</italic> (50.88%) encoded type III secretion system inner rod protein and needle protein, respectively. Multilocus sequence typing (MLST) showed considerable genetic diversity, with 34 distinct sequence types (STs) identified among 55 isolates. ST421 (<italic>n</italic> = 5), ST166 (<italic>n</italic> = 4), ST523 (<italic>n</italic> = 3), ST516 (<italic>n</italic> = 3), and ST507 (<italic>n</italic> = 3) were dominant STs among 55 <italic>V. alginolyticus</italic> isolates.</p></sec><sec><title>Discussion</title><p>These findings highlight the widespread occurrence of <italic>V. alginolyticus</italic> in both freshwater and seafood products, underscoring the critical need for vigilant monitoring of these bacteria. Such measures are essential for ensuring effective food safety management and safeguarding public health.</p></sec>