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ORIGINAL RESEARCH article

Front. Microbiol.
Sec. Microbiological Chemistry and Geomicrobiology
Volume 15 - 2024 | doi: 10.3389/fmicb.2024.1375808

The Role of Cattle Manure-Driven Polysaccharide Precursors in Humus Formation During Composting of Spent Mushroom Substrate

Provisionally accepted
萌萌 王 萌萌 王 宁 孙 宁 孙 Fengjun Yang Fengjun Yang *立琴 赵 立琴 赵 博文 范 博文 范 俊彩 刘 俊彩 刘 欣莹 孙 欣莹 孙 子铭 董 子铭 董
  • Heilongjiang Bayi Agricultural University, Daqing, China

The final, formatted version of the article will be published soon.

    The study examined the impact of adding cattle manure to the composting process of Agaricus bisporus mushroom substrate on compost humification. A control group CK comprised entirely of Agaricus bisporus mushroom substrate, while the experimental group CD (70 percent Agaricus bisporus mushroom substrate and 30 percent cattle manure) comprised the two composting treatments that were established. The study determined that the addition of cow dung has promoted the formation of humus components. Particularly, humic substance (HS-C) and humic acid (HA) increased by 41.3% and 74.7%, respectively, and the ratio of humic acid to fulvic acid (HA/FA) also increased by 2.78. It showed that the addition of cow dung accelerated the synthesis and decomposition of precursors, such as polysaccharides, polyphenols, and reducing sugars. thereby promoting the formation of humic acid. Network analysis revealed that adding cow dung promoted microbial interactions increased the complexity and stability of the bacterial and fungal symbiotic network, enhanced cooperation and reciprocity among microbes, and assisted in transforming fulvic acid (FA) components. Structural equation modeling (SEM) is a multivariate data analysis method for analyzing complex relationships among constructs and core indicators. SEM illustrated that introducing cattle manure into the composting process resulted in alterations to the correlation between physicochemical parameters and the microbial community, in addition to humus formation. Polysaccharides are the primary precursors for polymerization to form HA, which is an essential prerequisite for the conversion of fulvic acid to humic acid. Additionally, microbes affected the formation of humus, with bacteria substantially more influential than fungi. These findings provide new ideas for regulating the degree of humification in the composting process and have important practical implications for optimizing mushroom cultivation and composting techniques today.

    Keywords: composting, cattle manure, Spent mushroom substrate, humification, Precursors, Core microbes. 1

    Received: 24 Jan 2024; Accepted: 09 Jul 2024.

    Copyright: © 2024 王, 孙, Yang, 赵, 范, 刘, 孙 and 董. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Fengjun Yang, Heilongjiang Bayi Agricultural University, Daqing, China

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