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ORIGINAL RESEARCH article

Front. Microbiol.
Sec. Microbial Symbioses
Volume 15 - 2024 | doi: 10.3389/fmicb.2024.1374802
This article is part of the Research Topic Plant Microbiome: Interactions, Mechanisms of Action, and Applications, Volume III View all 11 articles

The functional characterization and microcosm evaluation of sorghum plant invigorating rhizobacteria

Provisionally accepted
Chiranjeevi M Chiranjeevi M 1Krishnaraj PU Krishnaraj PU 1Geetha D. Goudar Geetha D. Goudar 1NAGARAJU YALAVARTHI NAGARAJU YALAVARTHI 2*
  • 1 University of Agricultural Sciences, Dharwad, Dharwad, Karnataka, India
  • 2 Central Silk Board, Guwahati, India

The final, formatted version of the article will be published soon.

    The rhizosphere is enriched with diverse microflora, allowing for delving prospective microorganisms to enhance crop growth and yield for varied soil conditions. Demand for millet growth-promoting microorganisms is a contemporary need for dryland agriculture.Therefore, a detailed survey was conducted in northern Karnataka, India, to identify the millet growing areas, particularly Sorghum. The rhizobacteria from the Sorghum (Sorghum bicolor L.) were assessed for promoting seed germination using the paper-towel method and classified based on their efficiency. The elite isolates were positive for the Indole 3-acetic acid (IAA), Gibberellic acid (GA), phosphate, and zinc oxide solubilization, Hydrogen cyanide (HCN) production. The test isolates were antagonistic to Macrophomina phaseolina and Fusarium sp., and inhibited completely. Upon further evaluation, the cultures on Sorghum growth-promoting attributes under pot culture conditions, the plants inoculated with PG-152 (Bacillus subtilis) recorded the highest plant height, chlorophyll content, root dry weight, shoot dry weight, and total dry weight under ideal conditions of fertilization. Two isolates, PG-152 and PG-197, performing superior under pot culture conditions, were identified as Bacillus subtilis and PG-197 as Enterobacter sp., respectively, using 16S rDNA analysis. The sequences were allowed to screen open reading frames (ORF) and found several ORFs in Enterobacter cloacae and Bacillus subtilis, respectively. This study found that the rhizosphere is vital for identifying prospective isolates for biocontrol and plant growth-improving microorganisms.

    Keywords: Antagonistic Activity, IAA production, PGPR, Siderophore production, Sorghum

    Received: 22 Jan 2024; Accepted: 18 Jul 2024.

    Copyright: © 2024 M, PU, Goudar and YALAVARTHI. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: NAGARAJU YALAVARTHI, Central Silk Board, Guwahati, India

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